摘要
开展杉木优良无性系组织培养技术研究在生产上具有重要意义。文章开展了杉木外植体清毒、无菌材料增殖试验、无菌材料生根试验、瓶苗移栽等方面的研究,繁殖了一批苗木。试验结果表明,杉木不同无性系在同一培养基上增殖表现差异显著;杉木优良无性系I不定芽增殖比较好的配方是0.5 MS+6BA0.2mg/L+NAA0.2 mg/L,生根诱导较好的配方是GGR6 0.4 mg/L+根太阳1.2 mg/L;无性系D比较好的生根诱导配方是IBA0.4 mg/L+根太阳1.4 mg/L。
Developing tissue culture of chinese fir superior clones was important for forest wood production. The test on explant sterilization, sterile material proliferation and sterile materials rooting of chinese fir superior clones was reported in this paper. According on the test results, the differences of proliferation performance in the same medium was significant among different clones. The better formula for chinese fir superior clone Ⅰ is 0.5 MS ＋ 6BA 0.2 mg/L ＋ NAA 0.2 mg/L on proliferation of adventitious buds, and GGR6 0.4 mg/L ＋ sunroot 1.2 rag/ L on induced rooting. And the better induced root formula for superior clone D is IBA 0.4 mg/L ＋ sunroot 1.4 mg/L.
出处
《广东林业科技》
2009年第6期64-69,共6页
Forestry Science and Technology of Guangdong Province
基金
广东省自然科学基金"杉木优良无性系组培快繁技术研究"(5004951)
广东省林业种苗专项"杉木良种选育"
广东省林业科技创新专项"松杉良种选育和高效栽培技术研究与示范"(2008KJCX005-01
2009KJCX007-1)
关键词
杉木
优良无性系
组织培养
chinese fir, superior clone, tissue culture
