摘要
目的研究中国浙江省道地药材浙贝母的遗传多样性。方法应用扩增片段长度多态性技术(AFLP)对具有代表性的6个浙贝母居群,共32份个体进行分析。结果在浙贝母的物种水平上,Nei′s基因多样性指数(He)为0.169 0±0.175 7,Shannon′s信息指数(I)为0.269 8±0.245 3,多态位点百分率(PPB)76.85%;总遗传多样性(Ht)0.169 0±0.030 9,其居群水平上遗传多样性(Hs)0.150 8±0.024 0,Shannon′s信息指数(I)0.233 3±0.261 9,多态位点百分率(PPB)50.38%,Nei′s遗传分化指数(Gst)为0.107 6,基因流(Nm)为4.147 0。东阳和永康种群之间的遗传距离最小(0.015 0),而象山与缙云最远(0.032 4)。结论浙江省主产区栽培浙贝母物种的遗传多样性水平丰富,高的遗传多样性能够维持浙贝母的长期生存,居群间的多样性水平明显低于居群内的多样性水平,居群间遗传分化不明显,种植资源相对比较稳定,这些居群适宜作为浙贝母优良品种的选育基地。
Objective To study the genetic diversity of Fritillaria thunbergii, a traditional Chinese herb in Zhejiang Province in China. Methods The genetic diversity of six representational populations of F. thunbergii including 32 individuals was investigated by amplified fragment length polymorphism （AFLP） maker technique. Results The genetic diversity was revealed as follow： the Nei＇s genetic diversity index （He）0. 169 0±0. 175 7, Shannon＇s information index （I） 0. 269 8±0. 245 3, percentage of polymorphie loci （PPB） was 76.85% at the species level; Ht 0. 169 0±0. 030 9, and Hs . 150 8±0. 024 0, I 0. 233 3±0. 261 9, PPB was 50. 38% at population level. The genetic differentiation index （Gst） was 0. 107 6, Nm 4. 147 0. The result of dendrogram of six populations indicated that Dongyang and Yongkang populations shared the minimum genetic distance （0. 015 0）, they were classified into a group, and Xiangshan and Jinyun populations shared the maximum genetic distance （0. 032 4）. Conclusion The genetic diversity of F. thunbergii cultivated in Zhejiang Province is very rich, which could ensure the longterm survival of F. thunbergii. But the genetic diversity of F. thunbergii is relatively higher in population levels while lower at the species levels and the degree of genetic differentiation occured among the populations is not significant. The germplasm resources are relatively stable among these six populations. These populations could be used to breed the fine strains of F. thunbergii as the bases.
出处
《中草药》
CAS
CSCD
北大核心
2010年第1期109-113,共5页
Chinese Traditional and Herbal Drugs
基金
温州市科技局资助项目(Y2004A121)
关键词
浙贝母
种质资源
基因多样性
AFLP
Fritillaria thunbergii Miq.
germplasm resources
genetic diversity
amplified fragment length polymorphism （AFLP）
