摘要
根据GenBank中登录（登录号：AB115244）的鸭MHC-Ⅰα链序列设计并合成1对引物,采用RT-PCR技术从鸳鸯鸭脾脏组织中克隆出鸳鸯鸭MHC-Ⅰα链基因,并进行T-A克隆和序列测定。结果表明,所获得的鸳鸯鸭MHC-Ⅰα链基因长909bp,编码303个氨基酸的多肽,含一个完整的鸳鸯鸭MHC-Ⅰα链胞外区成熟肽基因。序列比较发现,鸳鸯鸭MHC-Ⅰα链基因与GenBank登录的其它品种的鸭MHC-Ⅰα链核苷酸同源性为89.4%～91.0%,与人和其他动物的MHC-Ⅰα链胞外区成熟肽基因的氨基酸同源性为11.1%～79.3%,表明MHC-Ⅰα链胞外区成熟肽基因存在着种的多样性,且亲缘关系越近,同源性越高。鸳鸯鸭MHC-Ⅰα链胞外区成熟肽基因的成功克隆为进一步研究鸭MHC-Ⅰ基因表达、生物学活性和应用奠定基础。
Yuanyang duck MHC class Ⅰα chain cDNA was completely amplified from a duck spleen by RT-PCR,then cloned and sequenced.The result showed cloned duck MHC class Ⅰα chain gene span is 909 bp,which encodes 303 amino acid residues,including a complete Yuanyang duck MHC-Ⅰα chain mature peptide（813 bp） of the extracellular domain.The results of homology analysis with other duck MHC-Ⅰα chain mature peptide gene of the extracellular domain revealed that there was identity of 89.4% to 91.0% at nucleotide homology.This study paved the way for future study of expression,biological function and application of duck MHC-Ⅰα chain mature peptide of the extracellular domain.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第12期60-63,共4页
China Animal Husbandry & Veterinary Medicine
基金
十一五国家科技支撑计划(2006BAD06A08)
