摘要
以指数流加策略高密度培养重组大肠杆菌,发酵生产胆固醇氧化酶。通过对比生长速率的分阶段控制使得细胞干重达40.128g/L。确定了最佳的诱导时机为菌体对数生长期的中期,产酶速率为1287.31U/(L·h)。菌体产酶水平达6436.56U/L,生产强度为459.75U/(L·h),实现了胆固醇氧化酶的高效生产。
Exponential fed-batch culture strategy were carried out to produce cholesterol oxidase （COD） using recombinant E. coli high cell-density culture. High cell-density was achieved by specific growth rate grading-control, and the final cell dry weight was 40.128g/L. The optimum induction time was determined as in the mid-logarithmic phase. Under the optimized cultivation conditions, the COD production rate was 1287.31U/（L · h）. The highest COD activity （6436.56U/L） and highest COD productivity [459.75U/（L·h）] were achieved.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2010年第1期130-133,共4页
Chemical Industry and Engineering Progress
基金
国家863计划资助项目(2006AA10Z305)资助
关键词
胆固醇氧化酶
高密度培养
指数流加
诱导时机
cholesterol oxidase
high cell-density culture
exponential fed-batch
induction time
