摘要
目的:探讨艾灸疗法调节慢性疲劳(chronic fatigue syndrome,CFS)的作用机制。方法:采用悬吊冷水游泳复合应激的方法复制CFS大鼠模型,艾灸组在复制模型同时艾灸双侧肝俞、脾俞、肾俞;通过Morris迷宫实验、鼠尾悬挂实验、力竭游泳实验进行行为学检测;采用放射免疫法检测下丘脑促肾上腺皮质激素释放激素(corticotrophin releasing hormone,CRH)、血浆促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)、皮质醇(cortisol,CORT)水平。结果:①模型组大鼠空间学习记忆力显著下降、力竭游泳时间显著缩短、悬挂不动时间显著延长,下丘脑CRH、血浆ACTH和CORT浓度显著升高,与正常组比较,差异有统计学意义(P<0.01)。②与模型组比较,艾灸组大鼠的行为学变化显著改善,下丘脑CRH、血浆ACTH和CORT水平显著降低(P<0.01)。结论:艾灸通过抑制下丘脑CRH,血浆ACTH、CORT的过度分泌,以纠正异常的HPA轴功能,达到治疗CFS目的。
Objective： To investigate the effects of moxibustion on ethological changes and hormones of hypothalamic-pituitary-adrenal （HPA） axis in chronic fatigue syndrome （CFS） model rats. Methods- The model of chronic fatigue syndrome （CFS） was induced by forced suspensory and cold water swimming stress. Rats in moxibustion group were treated with moxibustion at ＂ganshu＂, ＂pishu＂ and ＂shenshu＂ acupoints. Ethological changes were evaluated using Morris maze experiment, forced suspensory test and exhaustive swimming test. The levels of corticotrophin releasing hormone （CRH） in the hippocampus, adrenocorticotrophic hormone （ACTH） and cortisol （CORT） in plasma were assayed using radioimmunoassay method. Results： （1）Compared with those in normal control group, the ability of spatial learning and memory was significantly decreased, the time for exhaustive swimming and immobility of forced suspensory test was significantly shortened （P〈 0. 01）. The levels of hippocampus CRH, plasma ACTH and CORT were significantly increased （P〈0. 01）. （2）Compared with those in model group, the ethological changes were significantly improved, the levels of hippocampus CRH, plasma ACTH and CORT were significantly decreased. Conclusion： Moxibustion could modulate the excessive secretion of hippocampus CRH, plasma ACTH and CORT, and accordingly regulate the function of HPA axis, which might he the mechanism of moxibustion in treating CFS.
出处
《安徽中医学院学报》
CAS
2009年第6期43-46,共4页
Journal of Anhui Traditional Chinese Medical College
