摘要
神经干细胞移植治疗阿尔茨海默病鼠包括细胞替代治疗和基因治疗,神经干细胞移植阿尔茨海默病鼠脑后其组织形态学与行为学效应均可以得到不同程度的修复和改善。细胞替代治疗中,神经干细胞与神经营养因子联合移植效应优于单纯的神经干细胞移植,但目前对神经干细胞体内分化机制的不确定导致了神经干细胞移植治疗的盲目性,同时对影响其疗效的各种可能因素也缺乏比较研究。神经干细胞基因治疗具有细胞替代和基因治疗的双重作用,但尚处于研究的初期阶段,仍以NGF,BDNF,GDNF等单一营养因子基因修饰的神经干细胞移植治疗为主,且转基因神经干细胞移植入阿尔茨海默病鼠脑后外源基因表达效率、促分化、功能修复情况以及安全性的研究还很缺乏。目前神经干细胞移植治疗阿尔茨海默病鼠脑后的疗效检测技术手段比较单一,免疫组化方法与活体示踪技术的结合、形态学指标与功能学指标的综合检测是疗效检测的发展趋势。
There are two kinds of treatments on Alzheimer＇s disease （AD） rat by transplanting neural stem cells （NSCs）, Le. the replacing cell curing and the gene therapy. By replacing method, the AD rats showed signs of recovering to some extent on both histomorphology and behavior after transplanting NSCs into their brains. Transplanting NSCs along with the nerve nutrition factor （NTFs） showed better curative effects than NSCs transplantation alone. However, little is known about the molecular mechanism involving in the development of NSCs in vivo conditions. And the blindness of the treatment hindered the comparison of various affecting factors. The NSCs gene therapy is still in initial studying, with the effects of both cell replacement and gene therapy. This treatment genetically modified NSCs mainly by unitary nutrition fators such as nerve growth factor （NGF）, brain-derived neurotrophic factor （BDNF）, and glial cell line-derived neurotrophic factor （GDNF）. And it was almost known nothing about the exogenous gene expression efficiency, the induce differentiation, the restore of function and security after genetically-modified NSCs transplanted into the AD rat brain. The detecting technology of NSCs transplanting curative effects of the AD rat is unitary at present. And the combined method is the developing trend, such as combining the immunohistochemical method with in vivo-tracking, and combining morphology index with the function index.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第45期8987-8991,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
