摘要
目的:用超声提取法对鸡肉参中多糖提取工艺进行研究。方法:用正交实验设计法筛选最佳提取工艺,并用苯酚-硫酸法测定鸡肉参多糖含量。结果:λmax=491nm,平均回收率为102.05%,RSD为1.73%。鸡肉参多糖的最佳提取工艺为:超声提取时间120min,料液比为1∶40,提取次数为1次。结论:该方法简单、快速、准确,为从鸡肉参中提取多糖提供理论依据。
Objective： To study the optimum extraction process of polysaccharide from Incarvillea mairei（le＇vl） Grierson. Methods： The optimal ultrasonic extraction process was selected with the orthogonal design and the content of polysaccharide was determined by speetrophtometry. Result： The optimum extraction process was as follows： the polysaceharide was extracted by ultrasonic technology for 120 minutes, with 1：40 [S]/[L] ratio and extracted for once. The average recovery was 102.11%,RSD=1.78%,λmax = 491nm. Conclusion： The experimental results provide the basis for the ultrasonic extraction process of polysaceharide from Incarvillea mairei（le＇vl） Grierson. This optimum process is simple, stable and efficient.
出处
《大理学院学报（综合版）》
CAS
2009年第10期21-23,共3页
Journal of Dali University
基金
大理市科技局科研基金资助项目(2008SFS11)
关键词
鸡肉参
超声波提取
正交实验
lncarvillea mairei（le＇vl） Grierson
ultrasonic extraction process
orthogonal experimental design
