摘要
采用ＰＡＧＥ电泳法对成都麻羊的Ｈｂ、Ａｌｂ、Ｔｆ、Ｐｏ、Ａｍ、Ａｋｐ和Ｅｓ七个基因座的等位基因及ＬＤＨ的谱带与活力进行了检测．研究结果显示，成都麻羊的Ｈｂ基因座表现为单态，Ａｌｂ、Ｔｆ、Ｐｏ、Ａｍ、Ａｋｐ和Ｅｓ六个基因座表现出遗传多态性，得出了它们的基因型频率及基因频率；ＬＤＨ电泳显示５条谱带，其活力顺序为ＬＤＨ１＞ＬＤＨ３＞ＬＤＨ２＞ＬＤＨ５＞ＬＤＨ４．对Ａｌｂ、Ｔｆ、Ｐｏ、Ａｍ和Ｅｓ五个基因座上的基因型分布作适合性检验，发现这五个基因座上的基因型频率分布是平衡的．利用Ｈｂ、Ａｌｂ、Ｔｆ、Ｐｏ、Ａｋｐ、Ａｍ和Ｅｓ七个基因座上等位基因频率资料进行遗传变异分析，发现成都麻羊群体内基因纯合度高，杂合度低，遗传变异小．同时还利用Ｈｂ、Ｔｆ二个基因座上等位基因资料，对成都麻羊与国内其它１１个山羊品种（类型、品系）的亲缘关系进行了聚类分析，得出了聚类树系图．
The alleles in Hb、Alb、Tf、Po、Am、Akp and Es loci and enzyme zones of LDH and its activity of Chengdu Ma goat are studied by polyacrylamide gel electrophoresis.The studies show that,there is no polymorphism in Hb locus,the Alb、Tf、Po、Am、Akp and Es loci present genetic polymorphisms,and their genotype frequencies and gene frequencies are calculated.The LDH presents five enzyme zones,the sequence of their activities is LDH 1>LDH 3>LDH 2>LDH 5>LDH 4.The genetypic distributions of alleles in Alb、Tf、Po、Am and Es loci in population are tested by X 2 text,the results showing that genotypic distributions follow the Hardy Weinberg law in equilibrium.Using the data of allele frequencies in Hb.Alb、Tf、Po、Akp、Am and Es loci,genetic variation analysis show that genetic homozyosity in population is high,and heterogeneity is low,so Chengdu Ma goat has a low genetic variation in population.According to ablele frequencies in Hb and Tf loci of 12 goat breeds(types,strains) in our country,the clustering dendrogram map about the blood relationship of 12 goat breeds is obtained by cluster analysis.
出处
《西南民族学院学报（自然科学版）》
CAS
1998年第3期293-299,共7页
Journal of Southwest Nationalities College(Natural Science Edition)
