摘要
目的:找到一种简单、快速、准确的进行病毒性腹泻患者粪便标本中诺如病毒的检测方法,并对此方法进行评价。方法:用恒温快速检测方法进行154例北京地区病毒性腹泻患者粪便标本诺如病毒的检测,同时用逆转录-聚合酶链反应(RT-PCR)作对照,评价该方法。结果:在154例病毒性腹泻的病人标本中,采用恒温快速检测方法进行诺如病毒检测,阳性结果61例,阳性率为39.6%,RT-PCR方法进行检测,阳性结果54例,阳性率为35.1%,以RT-PCR为金标准计算,检测灵敏度90.7%(49/54),特异性88%(88/100)。这两组检验结果经过2χ检验,P=0.143,P值>0.05,说明两组结果之间差异无统计学意义,即两种实验方法结果无差异。通过一致性检验显示,KAPPA=0.765,P=0.000<0.05,说明这两种试验方法结果一致性较好。结论:恒温快速检测方法是一种简单、快速、准确的检测诺如病毒的方法,适合临床及流行病学筛查使用。
Objective：In order to find out and evaluate a more convenient,rapid and efficient way in detecting human Norovirus infections in stool specimens.Methods：Isothermal rapid amplification technique were used to detect human Norovirus in stool specimens collected from 154 human who all diagnosed as acute non-bacterial diarrhea.Reverse transcription-polymerase chain reaction（RT-PCR） was performed to evaluate this way.Results：Out of 154 patients with all diagnosed as acute non-bacterial diarrhea,61（39.6%） were positive for Norovirus RNA by isothermal rapid amplification technique,and 54（35.1%） were positive by RT-PCR.Compared with RT-PCR,the sensitivity and specificity of isothermal rapid amplification technique were 90.7%（49/54） and 88%（88/100） respectively.χ2 test showed that the differences were not significant（P0.05） and the consistency about two way was satisfactory by KAPPA test.Conclusion：To detect human Norovirus infection,isothermal rapid amplification technique seemed to be more convenient and time saving than RT-PCR which had been used worldwide.
出处
《中国卫生检验杂志》
CAS
2009年第11期2606-2607,共2页
Chinese Journal of Health Laboratory Technology
关键词
诺如病毒
恒温快速检测
RT-PCR
腹泻
Norovirus
Isothermal rapid amplification technique
RT-PCR
Diarrhea
