摘要
目的：研究青年人大肠癌抑癌基因ｐ５３蛋白的表达和细胞ＤＮＡ含量。方法：采用流式细胞光度术（ＦＣＭ）。结果：青年组大肠癌细胞ＤＩ值（１．３０±０．１７）显著大于老年癌组（１．１０±０．０９）（Ｐ＜０．０１），且细胞增殖指数（ＰＩ）亦明显大于后者（２４．９％±６．５％ｖｓ２０．２％±４．７％）（Ｐ＜０．０５）。青年组大肠癌中ＤＮＡ异倍体癌发生率为８７．１％（２７／３１），而老年癌组仅为２８．６％（４／１４），两者之间差别有高度显著性（Ｐ＜０．００１）。ｐ５３蛋白表达量青年患者（ＦＩ＝１．３４±０．２６）显著大于老年患者（ＦＩ＝１．１５±０．２５）（Ｐ＜０．０１）。在青年大肠癌中，粘液癌和侵犯周围软组织者，其ｐ５３蛋白的阳性表达率（１００％，９５％）分别高于腺癌和浸润较浅者（６７％，５８％）（Ｐ＜０．０５），而且低分化癌和有局部淋巴转移者ｐ５３蛋白的表达阳性率（９３％，１００％）也较高、中分化癌和无局部淋巴结转移者（６９％，６８％）为高。结论：在ＤＮＡ水平上，青年大肠癌的恶性程度高于老年大肠癌；ｐ５３蛋白的高表达可能是造成青年大肠癌恶性度高的原因之一。
Purpose To study the expression of p53 protein and DNA content in colorectal cancer of young adults. Methods by Flow Cytometry. Results Cancer cell DI value of young group (1 30±0 17) was higher than that of elder group (1 10±0 09) significantly ( P <0 01) and so was proliferation index ( PI )(24 9%±6 5% vs 20 2%±4 7%) ( P <0 05). The percentage of DNA aneuploidy tumor was 87 1% (27/31) in young group, but 28 6%(4/14) in elder group, there was very significant deference between them ( P <0 001). Cancer cell FI value of young patients (1 34±0 26) was higher than that of elder patients (1 15±0 25) significantly ( P <0 01). Moreover, p53 positive percentage of mucinous carcinoma and cancer with soft tissue involved (100%, 95%) were higher than adenocarcinoma and cancer without soft tissue involved (67%, 58%) in young patients, respectively ( P <0 05). There was a trend that p53 positive expression of poor differentiated and cancer with lymph node metastasis was higher than well moderate differentiated and cancer without lymph node metastasis, respectively. Conclusion The colorectal carcinoma in the young patients is more malignant than that in elder patients at DNA level. Overexpression of p53 protein is one of important reasons for high malignancy of young patients with colorectal carcinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
1998年第5期475-477,共3页
Chinese Journal of Clinical and Experimental Pathology
