摘要
最近的一项研究报导,采用流式细胞仪分选技术从人胃癌细胞株中分离出CD44+胃癌干细胞.20～30×103个CD44+细胞入NOD/SCID鼠腹部皮下和胃浆膜下能形成胃癌移植瘤,100×103个CD44-的细胞入NOD/SCID鼠体内不形成肿瘤.采用无血清、无粘附间质的干细胞体外培养方法,发现CD44+的细胞能形成肿瘤微球体,具有自我更新能力,而CD44-的细胞则不形成球形克隆.上述的实验结果说明,在人胃癌细胞株中存在胃癌肿瘤干细胞.据此可以相信,胃癌干细胞是胃癌细胞中具有自我更新及分化潜能的一小群细胞,不能被目前的化疗、放疗等抗癌治疗措施所杀灭,是胃癌术后复发、肿瘤进展扩散转移的根源.胃癌干细胞可能来源于骨髓干细胞.随着对胃癌肿瘤干细胞生物学研究的深入,必将为胃癌的临床诊断和治疗提供新的策略.
A recent study identified gastric cancer stem cells （CSCs） by isolating CD44^＋ and CD44^- subpopulations from human gastric cancer cell lines using fluorescence-activated cell sorting （FACS）. Only CD44^＋ cells, but not CD44^- cells from gastric cancer cells displayed tumorigenic capacity when these cells were subcutaneously and orthotopically injected into non-obese diabetic severe combined immunodeficient （NOD-SCID） mice. In vitro, it has been demonstrated that CD44^＋ ceils showed spherical colony formation in serum-free conditions. The growth of tumor spheres is considered as an indicator of self-renewal ability, the key characteristic of CSCs. CD44^- gastric cancer cells formed few or no colonies. These data strongly support the existence of gastric CSCs. Based on this study, we believe that gastric cancer stem cells are small subpopulations of gastric cancer cells, which can both self-renew and produce differentiated progeny, they are resistant to chemotherapy and radiation. It has been reported that bone marrow-derived cells （BMDC） are the possible origin of the gastric cancer stem cells. The expanding research of gastric cancer stem cell biology may lead to a novel diagnosis and treatment of gastric cancer.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2009年第10期889-895,共7页
Chinese Journal of Biochemistry and Molecular Biology
基金
重庆市自然科学基金资助(CSTC
No.2009BB5017)~~
关键词
胃癌细胞
肿瘤干细胞
肿瘤治疗
gastric cancer cell
cancer stem cells
cancer therapy
