摘要
目的研究核因子κＢ（ＮＦκＢ）对白细胞介素１β（ＩＬ１β）引起的体外培养的人肾小球系膜细胞表达细胞间粘附分子（ＩＣＡＭ１）的基因调控机理。方法采用凝胶迁移率法观测ＮＦκＢ的活化，以Ｎｏｒｔｈｅｒｎ杂交方法检测细胞间粘附分子的基因表达，用细胞ＥＬＩＳＡ法检测ＩＣＡＭ１蛋白水平的表达。结果ｒｈＩＬ１β１０ｎｇ／ｍｌ刺激肾小球系膜细胞１、２、４小时，均引起ＮＦκＢ活化，且１小时为最强。４小时，ＩＣＡＭ１ｍＲＮＡ表达水平上调０３５（刺激前为０１４）。结论细胞因子ＩＬ１β刺激人肾小球系膜细胞表达细胞间粘附分子是通过ＮＦκＢ调控，ＮＦκＢ可能参与调节肾小球疾病的免疫炎症的过程。
Objective To study the regulative effect of nuclear factor κB (NF κB) on the expression of intercellular adhesion molecular 1(ICAM 1) in human mesangial cells (HMC) by interleukin Ⅰ 1β (IL 1β). Methods Activation of NF κB was measured by electrophoresis mobility shift assay (EMSA). ICAM 1 expression was detected by Northern Blot and Cell ELISA. Results rhIL 1β (10 ng/ml) could rapidly stimulate activation and translocation of NF κB, and also could enhance the expression of ICAM 1 in mRNA (0.14vs 0.35) and protein (0.92±0 10 vs 1.35±0.11, P <0.01) level. After pretreatment with 100 μmol/L L 1 chlor 3 (4 tosylamido) 4 phenyl 2 butanon (TPCK), an inhibitor of NF κB, both ICAM 1 levels of mRNA and protein stimulated by rhIL 1β were blocked by about 50% in these cells (0.46±0.05 vs 1.29±0.12, P <0.01) compared with the rhIL 1β stimulated group. Conclusion These results suggest that NF κB is one of the signaling factors for IL 1 stimulated ICAM 1 expression in HMC. It may modulate the immune inflammatory process in glomerular diseases.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1998年第4期290-292,共3页
National Medical Journal of China
基金
国家自然科学基金
