摘要
目的探讨邻苯二甲酸二丁酯(di-n-butyl phthalate,DBP)和邻苯二甲酸二(2-乙基己基)酯(di-2-ethy lhexyl phthalate,DEHP)对雄性大鼠精子生成的联合毒性作用。方法将32只健康清洁级成年SD雄性大鼠按体重随机分为4组,分别为阴性对照组(玉米油)、DBP染毒组(1/20LD50,1.0g/kg)、DEHP染毒组(1/20LD50,1.7g/kg)和DBP+DEHP染毒组(1.0g/kgDBP+1.7g/kgDEHP),每组8只。采用灌胃方式进行染毒,每天1次,每周5d,连续染毒8周。末次染毒24h后,测量大鼠体重、睾丸和附睾及肝脏脏器湿重,测定精子参数(精子数量、精子活动率及精子畸形率),并观察睾丸及附睾组织形态学改变。结果DBP和DEHP联合染毒对大鼠体重增长无交互作用(P>0.05);对睾丸和附睾及肝脏脏器系数存在交互影响,使睾丸和附睾的脏器系数明显降低,肝脏的脏器系数明显升高,表现为协同作用;对精子计数、精子活动率及精子畸形率存在交互影响,使精子数量和存活率明显降低,畸形率明显升高,表现为协同作用。光镜下可见DBP和DEHP联合染毒可引起曲细精管外形不规则、萎缩变性,间质增宽,生精上皮退化变性,甚至几乎消失,生精细胞耗竭,基底部仅有少量的支持细胞且细胞肿胀、变性,出现空泡样改变,基膜尚完整;附睾上皮受损且变薄,间质增宽,管腔内几乎不见成熟精子。结论DBP和DEHP联合染毒对雄性大鼠具有明显的生殖毒性作用,可严重影响精子生成数量和质量,且对附睾有一定的毒作用。
Objective To study the combined effects of di-n-butyl phthalate（DBP） and di-2-ethylhexyl phthalate（DEHP） on spermatogenesis of male rats. Methods Thirty-two healthy and adult male SD rats were randomly divided into 4 groups,8 rats in each,including a control group （given coin oil） and three experimental groups： DBP （1/20 LD50, 1.0 g/kg,dissolved in coin oil）, DEHP （1/20 LD50, 1.7g/kg,dissolved in coin oil） and DBP＋DEHP （1.0 g/kg＋1.7 g/kg,dissolved in coin oil）,the treatment was conducted through gavage, once a day, five days a week for 8 consecutive weeks. Body weight gain and testis, epididymis and liver organ weights were determined and the organ coefficients were counted. Sperm parameters were determined （sperm counts, the spermatozoon survival rate and the rate of the sperm deformation）. The histomorphological examination of the testis and epididymis was conducted. Results There was no interaction between DBP and DEHP on the body weight （P〉0.05）. DBP and DEHP obviously induced a decrease in organ body weight ratios of testis and epididymis and an increase in organ body weights ratios of liver. Obvious decrease in the sperm counts, spermatozoon survival rate and significant increase in the rate of the sperm deformation were observed. There was synergism between DBP and DEHP on the testis, epididymis and liver organ body weight ratio, as well as sperm counts, sperm survival and deformation rates. Pathological examination showed the seminiferous tubules were irregular shape, degenerative atrophy and interstitial substance broadening,and the seminiferous epithelium were degeneration. Epididymis epithelium was damaged and few of mature sperm was seen. Conclusion DBP combined with DEHP can cause obvious toxic effects on reproductive function in male rats. DBP and DEHP mixture can strongly affect the sperm quantity and quality. Also, some toxic effects to epididymis are observed.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第10期853-856,共4页
Journal of Environment and Health
基金
国家自然科学基金资助项目(30760208)
