摘要
为阐明活性氧(ROS)对睾丸支持细胞(SC)的影响,并深入探讨精子发生调节机制,通过体外培养的仔猪睾丸支持细胞研究ROS对SC中微丝结构的影响。利用H2O2模拟细胞内的ROS环境,使用细胞松弛素B(CytB)破坏细胞中的微丝并利用维生素E(VE)抑制细胞内的ROS,通过细胞活性检测、免疫荧光、酶活检测、免疫印迹等方法检测ROS对睾丸支持细胞微丝的影响。结果表明,H2O2和细胞松弛素B均能提高SC内ROS的水平和细胞外信号调节激酶(ERK)的活性,降低抗氧化酶的活性,破坏细胞内微丝的结构和分布,二者具有协同作用,VE能降低ROS对SC的作用。上述证明,H2O2通过不依赖于微丝途径和降低细胞内抗氧化能力使细胞内ROS处于较高水平,并通过ERK级联,使细胞中微丝发生多聚化,破坏微丝的结构。
To elucidate the effect of reactive oxygen species（ROS） on Sertoli Cell and provide some fundamental data for the study of mechanism of spermatogenesis,Sertoli Cell of piglets was chose to study the effect of ROS on microfilaments in Sertoli Cells.Sertoli Cells were treated with H2O2 and cytochalasins B,and vitamin E was used as an antioxidant.Cell viability,antioxygen enzymes activity and changes of activity of ERK1/2（extracellular signal-regulated kinase） were detected.The results showed as followed： H2O2 and cytochalasins B could increase the level of ROS and activity of ERK1/2 of cells,decrease antioxygen enzymes ability,and destroy the structure and distribution of microfilaments.H2O2 and cytochalasins B had synergistic effect on the level of ROS in Sertoli Cells,while Vitamin E decreased the effect of ROS on cell microfilaments.The results suggested that H2O2 resulted in the increase of ROS of Sertoli Cells by microfilament-independence way and the decrease of antioxygen ability;the high level of ROS destroyed cell microfilaments through activating ERK.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第9期1297-1302,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(30270955)
关键词
活性氧
睾丸支持细胞
微丝
reactive oxygen species
Sertoli Cell
microfilaments
