摘要
采用金属配位剂Ni（Ⅱ）-NH3作为衍生剂,与游离氰化物反应生成稳定且具有高紫外吸收值的Ni（CN）42-,使用高效液相色谱-紫外检测法（HPLC-UV）检测游离氰化物。方法的线性范围为0.014-0.540 mg/L,相关系数R2=0.9984,检出限为4.0μg/L。该方法简便快速,抗干扰能力强,灵敏度高,适于食品、环境、生物等样品中游离氰化物的检测。
A new method involving metal-ligand reagent derivatization and high-performance liquid chromatography（HPLC） was developed for the determination of free cyanide in grain and distilled spirit.The free cyanide reacted with Ni（Ⅱ）-NH3 to form a stable and highly UV absorbing tetracyanonickelate anion,and then was determined by HPLC.The calibration curve was linear for CN-in the range of 0.014-0.540 mg/L（R^2=0.9984）.The detection limit（S/N=3） was estimated to be 4.0 μg/L for CN^-.This method is simple,rapid,low interference and with high sensitivity, which can be used for the determination of free cyanide in common food, environmental and physiological samples.
出处
《分析科学学报》
CAS
CSCD
北大核心
2009年第5期587-589,共3页
Journal of Analytical Science
关键词
游离氰化物
衍生化
高效液相色谱
粮食
白酒
Free cyanide
Derivatization
High-performance liquid chromatogrphy
Grain
Distilled spirit
