摘要
从患软壳综合症的濒死青虾体内分离到一株细菌QXL0711B,经人工感染试验,其对青虾的半数致死浓度(LC50)为1.47×106CFU/mL,具有较强毒力。API32E系统鉴定及16SrRNA序列分析,该病原菌为维罗纳气单胞菌温和生物变种(Aeromonas veronii biovar sobria,登录号:FJ808727)。其系统发育分析表明,菌株QXL0711B与维罗纳气单胞菌(登录号:X71120)和维罗纳气单胞菌温和生物变种(登录号:AY987729)的亲缘关系最近,其同源性都为99%。药敏实验结果表明:菌必治、复达欣、环丙沙星、恩诺沙星、氟哌酸等5种化学疗剂对该病原菌有较强的抑菌作用。
A pathogenic bacterial strain QXL0711B was isolated from freshwater shrimp （Macrobrachium nipponense） suffering with soft-shell syndrome. The 50% lethal concentration （LC50） of strain QXL071 I B was 1.47×10^6 CFU/mL, which showed that strain QXL071 I B was rather strong virulent to Macrobrachium nipponense. Strain QXL07 l lB was gram negative and had β-hemolytic activity on sheep blood agar. By means of API 32E identification and 16S rRNA sequence analysis, strain QXL0711B was identified as Aeromonas veronii biovar sobria （locus number： FJ808727）, which was the closest relative to Aeromonas veronii strain （locus number： X71120） and Aeromonas veronii biovar sobria strain （locus number： AY987729） with 99% homology. In addition, strain QXL0711B was highly sensitive to ceftriaxone ceftazidime ciprofloxacin enrofloxacin and norfloxacin.
出处
《微生物学通报》
CAS
CSCD
北大核心
2009年第10期1571-1576,共6页
Microbiology China
基金
浙江省科技计划项目(No.2008C22058)
湖州市科技计划项目(No.2007YN22)
德清县科技合作专项
关键词
青虾
软壳综合症
维罗纳气单胞菌
日本沼虾
Freshwater shrimp, Soft-shell Syndrome, Aeromonas veronii, Macrobrachium nipponense
