摘要
目的:从老参地土壤中分离得到一株对西洋参锈腐病菌具有良好拮抗作用的菌株F05,对该菌株的发酵培养基进行了初步研究,确定了其最佳培养基组成。方法:利用单因素试验和均匀设计试验优化培养基组分。结果:优化结果碳源为玉米秆粉,氮源为磷酸氢二铵,无机盐为硫酸镁、磷酸氢二钾,质量分数分别为3.720 6%,0.531 2%,0.035 5%,0.040 0%,优化后活菌生物量为1.57×109CFU.mL-1。
One strain F05 which had better antagonism for American ginseng rust rot was obtained from continuous cropping ground,and its fermentation had been preliminarily studied,more over the research can further determine the optimum composition.The single factor and uniform design were used to optimize the formulation medium.The identified formulation was powder of cornstalks 3.720 6%,（NH4）2HPO4 0.531 2%,MgSO4 0.035 5%,K2HPO4 0.040 0%.The bacteria number was 1.57×10^9 per milliliter culture solution.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第18期2296-2298,共3页
China Journal of Chinese Materia Medica
基金
国家"十一五"科技支撑计划子课题(2006BA109804-02)
关键词
发酵
优化
放线菌
均匀设计
复配
fermentation
optimization
antagonistic
uniform design
combination
