摘要
目的:探讨新生儿IL-18对Th1/Th2类细胞免疫平衡的作用与HBV宫内感染的相关性。方法:选择HBsAg、HBeAg双阳性但肝功能正常孕产妇分娩的新生儿157例,以其中胎儿宫内感染21例为研究组,无宫内感染136例为对照组。用流式细胞仪检测两组脐血白细胞介素-18(IL-18)、γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)的表达水平。结果:(1)研究组IL-18及IFN-γ表达水平明显低于对照组,差异有显著性(P<0.05;P<0.05);研究组IL-4表达水平百分数高于对照组,但差异无显著性(P>0.05);研究组IFN-γ/IL-4高于对照组,差异有显著性(P<0.05);(2)研究组IL-18与IFN-γ、IL-4、IFN-γ/IL-4均呈显著正相关(P均<0.05)。结论:IL-18是Th1/Th2网络平衡的更高层次的调控者;IL-18水平下调,导致新生儿Th1/Th2细胞因子比例下降,以致不利于HBV的清除,IL-18水平下调可能是HBV宫内感染的危险因素之一。
Objective：To study the relationshipbetween the effects on immune balance of T helper cell 1,2（Th1/Th2） by interleukin-18 （IL-18）level of newborns and hepatitis B vires infection. Methods：One hundred and fifty-seven full-term neonates born by HBsAg and HBeAg postive mothers whose liver function were normal were selected. The intrauterine infection group was consisted of 21 cases for neonates and the non-intrauterine infection group was consisted of 136 cases for neonates. The two groups of newborns were both detected for interleukin-18,interferon-γ,interleukin4 of cord blood by flow cytometry. Results： （ 1 ）The percentage of IL-18,IFN-γ levels of cord blood in study group was significantly lower than that of control group（P〈0.05） ;The mean eytokine IL-4 levels of cord blood in study group was higher than that of the control group（P〉0.05）. The ratio of the cord blood IFN-γ/IL-4 levels of the cord blood in study group was significantly higher than that of the control group（P〈0.05 ） ;（2）The positive correlation existed between IL-18 and IFN-γ, IL4, IFN-γ/IL-4 both in two groups. Conclusion：IL-18 regulated immune balance of Th1/Th2, and the decrease of the cord blood IL-18 maybe induce to the decrease of ratio of Th1/Th2 type cytokines in the cord blood. The imbalance of immune regulation of Th1/Th2 maybe the main mechanism of HBV intrauterine infection ;HBV intrauterine infection is related to the decrease of IL-18.
出处
《现代妇产科进展》
CSCD
北大核心
2009年第9期678-681,共4页
Progress in Obstetrics and Gynecology
基金
江西省卫生厅科研立项项目(No:20061144)
