摘要
本实验以清洁级ICR雄性小白鼠为实验动物,研究了不同浓度缢蛏对60Co γ射线辐照致雄性小白鼠遗传损伤的修复作用。6～7周龄小白鼠辐射后分别灌胃100mg/(kg·d)、200mg/(kg·d)、400mg/(kg·d)剂量的缢蛏10天。采用小白鼠骨髓嗜多染红细胞(polychromatic erythrocytes,PCE)微核实验、小白鼠精子畸形实验等方法,测定小白鼠胸腺指数及脾指数、骨髓有核细胞数及PCE微核率、小白鼠精子畸形率等指标。实验结果表明:3个剂量缢蛏组均能使由60Co γ射线辐照引起的小白鼠胸腺指数及脾指数、骨髓有核细胞数下降得到显著回升(P<0.001);3个剂量缢蛏组均能使由60Co γ射线辐照引起的小白鼠PCE微核率及精子畸形率升高显著下降(P<0.001)。因此,实验剂量的缢蛏对60Co γ射线辐照引起的雄性小白鼠的遗传损伤具有明显的修复作用。
We studied the repairing effect of Sinonovacula constricta on genetic injury of ICR male mice induced by ^60Coγ-rays. Mice with 6-7 week age were irrigated respectively by 100 mg/（kg·d）, 200 mg/（kg·d）, and 400 mg/（kg·d） Sinonovacula constricta for 10 d. PCE micronucleus test and sperm deformity test were used to measure the index of spleens and chest glands, the number of karyote and the frequency of PCE micronucleus, and the frequency of sperm deformity. The result showed that the 3 different concentrations of Sinonovacula constricta made increase significantly the index of spleens and chest glands, the number of karyote that had been decreased by ^60Coγ-rays （P〈0.001）. The three different concentrations of Sinonovacula constricta made decrease significantly the frequency of PCE micronucleus and sperm deformity decreased by ^60Coγ-rays （P〈0.001）. The conclusion is that the test concentrations of Sinonovacula constricta have obvious repairing effect on the genetic injury of male mice radiated by ^60Coγ-rays.
出处
《细胞生物学杂志》
CAS
CSCD
2009年第4期575-579,共5页
Chinese Journal of Cell Biology
基金
乐清市科技局科研基金资助项目(No.2004R024)~~
关键词
缢蛏
^60Coγ射线
遗传损伤
修复作用
Sinonovacula constricta
^60Coγ-rays
the genetic injury
repairing
