摘要
目的观察血小板衍生生长因子（PDGF）对肝星状细胞c—jun基因表达的影响。方法在培养的肝星状细胞株中加入不同浓度的PDGF（终质量浓度8、40、200ug/L），于8、24、48、72h4个时间点分别收集细胞，提取肝星状细胞总RNA；用逆转录一聚合酶链反应（RT-PCR）方法测定c-jun的基因表达水平。结果PDGF3组HSC—T细胞c-jun基因表达水平在8h（1．71±0．20、2．30±0．26、3．30±0．29）、24h（1．46±0．13、1．98±0．19、2．73±0．23）、48h（1．11±0．10、1．63±0．16、2．32±0．21）、72h（0．77±0．09、1．23±0．10、1．91±0．16）4个时间点均显著高于对照组；随剂量加大，HSC-T6细胞c-jun基因表达水平逐渐升高；3组间差异有统计学意义（P〈0．01）。结论PDGF可显著增强肝星状细胞c—jun的基因表达。
Objective To investigate the effects of platelet growth factor （PDGF） on c-jun gene expression in hepatic stellate cells. Methods Hepatic stellate cells （HSC-T6） were cultured in medium containing different concentrations of PDGF （ 8,40,200ug/L respectively）, and HSCs were harvested at different incubation periods （8,24,48,72 h）. Total RNA of HSCs was isolated ,and the c-jun gene expression level was detected by reverse transcription polymerase chain reaction. Results The c-jun gene expression level of HSCs in low （8 ug/L） ,middle （40ug/L） and high （200 tLg/L） PDGF groups was significantly higher than in control group after exposure to PDGF at 8 h （ 1.71 ± 0.20,2.30 ± 0.26,3.30 ± 0.29） ,2d h （1.46 ±0.13,1.98 ±0.19,2.73 ±0.23） ,48 h （1.11 ±0.10,1.63±0.16,2.32±0.21） and 72 h （0.77 ± 0.09,1.23 ± 0. 10, 1.91 ± 0. 16）, respectively. The c-jun gene expression levels in HSCs were enhanced gradually with the increased dosage of PDGF. There was significant difference in the c-jun gene expression among three PDGF groups. Conclusion PDGF may strongly up-regulate the c-jun gene expression in HSCs.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第9期1153-1154,共2页
Chinese Journal of Experimental Surgery
基金
军队“十五”杰出人才基金资助项目（01J002）
