摘要
目的研究甲氰咪呱(cimetidine)对K562细胞体外诱导脐血T细胞TCR Vβ亚家族克隆性的影响。方法体外分别将甲氰咪呱、K562细胞、甲氰咪呱+K562细胞与正常人的脐带血单个核细胞(mononuclear cells,MNC)混合培养2周,检测诱导增殖T细胞的特异杀伤活性,同时利用RT-PCR基因扫描技术分析培养后T细胞的TCR Vβ亚家族谱系的选择性利用和克隆性增殖情况。结果经甲氰咪呱、K562细胞、甲氰咪呱+K562细胞诱导培养2周后,各组均不同程度诱导细胞增殖,其中甲氰咪呱联合K562细胞诱导组的T细胞对K562细胞的特异性杀伤作用显著增强(P<0.01)。3组均呈现不同程度的T细胞TCR Vβ亚家族选择性利用,均有TCR Vβ21亚家族呈克隆性增殖。结论甲氰咪呱可协助增强bcr-abl抗原刺激的特异性抗CML T细胞作用。
Objective To investigate the effect of cimetidine on the clonal expansion of TCR Vβ subfamily of T cells in cord blood after the stimulation by K562 cells in vitro. Methods Cimetidine （ 1 × 10^-5mol/L） or K562 cells （1 × 10^6/ml） or both of them were respectively cultured with mononuclear cells （MNC） isolated from normal human cord blood for 2 weeks. After the induction, specific cytotoxicity of the proliferated T cells were detected with K562 cells as the target cells. The selective usages and clonal expansion of TCR Vβ subfamily of T cells were analyzed by RT-PCR and genescan technique. Results After induction for 2 weeks, the 3 groups showed the increased cell proliferation, in which specific eytotoxicity of T cells induced by both cimetidine and K562 cells against K562 cells was enhanced significantly compared with the other 2 groups （P 〈 0. 01 ）. Differental selective usages of T cells TCR Vβ subfamily and TCR Vβ21 clonal proliferation were found significantly in every group. Conclusion Cimetidine enhances the specific cytotoxicity of T cells stimulated by chronic myelogenous leukemia L antigen
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第17期1637-1640,共4页
Journal of Third Military Medical University
基金
国家高技术研究发展计划(863计划)(2006AA02Z114)
广东省自然科学基金(06025169)
暨南大学病理生理重点实验室开放课题基金(2006)~~
