摘要
目的:观察兔肾缺血再灌注损伤时血栓素/前列环素体系的动态变化,探讨中药川芎嗪注射液的调控作用及机制。方法:日本大耳白兔30只,随机均分为3组:假手术组、再灌注组、川芎嗪组。复制在体兔肾缺血再灌注损伤动物模型,在缺血前、缺血1 h、再灌注1、3、5 h分别经颈总动脉抽血检测血栓素B2(TXB2)和6-酮-前列腺素F1α(6-keto-PGF1α)含量,计算其比值;实验结束时取肾组织检测TXB2和6-keto-PGF1α含量与比值。结果:再灌注组血浆TXB2含量随缺血和再灌注时间的延长呈阶梯性升高,与假手术组各时点相比均明显升高(均P<0.01);川芎嗪组血浆TXB2含量与再灌注组及假手术组同时相点比较,均明显下降(均P<0.01)。川芎嗪组血浆TXB2/6-keto-PGF1α在缺血和再灌注各时点与假手术组相比差异均无统计学意义(均P>0.05),但均显著低于再灌注组(均P<0.01)。川芎嗪组与再灌注组比较肾组织TXB2、TXB2/6-keto-PGF1α下降非常明显(均P<0.01),6-keto-PGF1α差异无统计学意义(P>0.05)。结论:川芎嗪能抑制血小板释放TXA2,调控TXA2/PGI2的平衡,遏制无复流现象,故可减轻肾缺血再灌注损伤。
AIM： To investigate the dynamic change of thromboxane/prostacyclin in renal ischemia reperfusion injury rabbits, and approach the regulatory effects of ligustrazine injection on renal ischemia reperfusion injury and its mechanisms. METHODS： Thirty rabbits were divided into three groups randomly： sham operation group （group S）, ischemia reperfusion injury group （group IR） and ischemia reperfusion injury plus ligustrazine injection group （group LZ）, each group had ten rabbits. The renal ischemia reperfusion injury model of rabbit was established in vivo. The content of thromboxane B2 （ TXB2 ）, 6-keto-prostaglandin F1α （6- keto-PGF1α） were detected in the blood plasma gathered from common carotid artery at times： pre-ischemia, ischemia 1 h, reperfusion 1, 3 and 5 h,the ratio of TXB2 and 6-keto-PGF1α were calculated. The content and the ratio of TXB2 and 6-keto-PGF1α in kidney tissue were detected at the end of the experiment. RESULTS： The content of TXB2 in plasma showed a time-dependent ascensus in group IR during ischemia reperfusion, it was significant higher than that in group S at all time points （all P 〈 0.01 ）. Compared with-group IR and with group S, the content of TXB2 in plasma was decreased significantly in group LZ at each time point during ischemia reperfusion （all P 〈 0.01 ）. Compared with group S, there was not different significance for the TXB2/6-keto-PGF1α in group LZ（all P 〉 0.05）. Compared with group IR, it was significant lower in group LZ at each of time point （all P 〈 0.01 ）. Compared with group IR, in group LZ, the TXB2 level and TXB2/6-keto-PGF1α in kidney tissue were de-creased remarkably （all P 〈 0.01）, there was no different significances in 6-keto-PGF1α （P 〉 0.05 ）. CONCLUSION： Ligustrazine could inhibit platelet to release TXA2, regulate the balance of TXA2/PGI2, restrain no re/low phenomenon, so it could prevent renal ischemia reperfusion injury.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2009年第6期637-641,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
浙江省教育厅科研基金资助项目(19990444)
关键词
川芎嗪
肾
缺血再灌注损伤
血栓素A2
前列环素
ligustrazine
kidney
ischemia reperfusion injury
thmmboxane A2
prostacyclin
