期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

聚乙二醇琥珀酰亚胺酯修饰牛血红蛋白作为红细胞代用品的条件研究 被引量：1

Research on Prerequisite for Using Bovine Hemoglobin Modified by Polyethylene Glycol as a Red Blood Cell Substitute
原文传递
导出
摘要 以单甲氧基聚乙二醇甲酸琥珀酰亚胺酯(Succinimidyl carbonate methoxy polyethylene glycol,SC-mPEG)为修饰剂对牛血红蛋白(Bovine hemoglobin,BHB)进行了修饰,考察了不同条件下,SC-mPEG与牛血红蛋白反应的产率,并且建立了SC-mPEG-BHB混合物的分析方法。根据不同反应因素对SC-mPEG修饰血红蛋白反应的影响确定了SC-mPEG与牛血红蛋白反应的最佳条件,即SC-mPEG与牛血红蛋白最佳配比为60∶1,在4℃,pH9的缓冲溶液体系反应2h。 This paper aims to raise concerns about the modification of bovine hemoglobin with the use of SCmPEG as modifying reagent. The yield rates of SC-mPEG-BHB under dissimilar conditions are studied, and more important, a series of methods used to characterize the SC-mPEG--BHB mixtures are built. The best reactive system has been confirmed, i.e. at 4℃, pH9; SC-mPEG and BHB are mixed according to the proportion of 60： 1 and be submitted to reaction for 2 h.
作者 孙居锋 刘云艳
机构地区 滨州医学院药学院 烟台山医院
出处 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2009年第4期815-819,共5页 Journal of Biomedical Engineering
关键词 单甲氧基聚乙二醇甲酸琥珀酰亚胺酯 聚乙二醇 牛血红蛋白 红细胞代用品 Succinimidyl carbonyl methoxy polyethylene gIycol（SC-mPEG） Polyethylene glycol Bovine hemoglobin（BHB） Red blood cell substitutes
分类号 R91 [医药卫生—药学]
  • 相关文献

参考文献9

  • 1王雁峰,潘继伦,俞耀庭.红细胞代用品的研究进展[J].生物医学工程学杂志,2004,21(3):490-494. 被引量：4
  • 2TALARICO T L, GUISE K J, STACEY C J. Chemical characterization of pyridoxalated hemoglobin polyoxyethylene conjugate[J]. Biochim Biophys Aeta, 2000,1476 (1):53-65.
  • 3SAKAI H, TAKEOKA S, TSUCHIDA E. Purification of concentrated hemoglobin using organic solvent and heat treatment[J]. Protein Expr Purif, 1993,4:563-569.
  • 4PEREIRA D, TONTHAT HAI, NELSON D. A convenient large scale synthesis of N, N"-disuccinimidyl carbonate [J]. Synthetic Communications, 1998,28(21): 4019-4024.
  • 5MIRON T, WILCHEK M. A simplified method for the preparation of Suecinimidyl Carbonate polyethylene Glycol for coupling to proteins [J]. Bioconjugate Chemistry, 1993, 4: 566- 569.
  • 6白坚石,卜凤荣,李松,胡远东.聚乙二醇修饰牛血红蛋白制备红细胞代用品的初步研究[J].中国生物制品学杂志,2002,15(2):97-100. 被引量：10
  • 7HARUHIRO M, KAORU S, YASUHISA Y, et al. Detection of serum proteins by native polyaerylamide gel eleetroph-oresis using Blue Sepharose CL-6B-containlng stacking gels [J]. Analytical Biochemistry, 2002,307 (2) : 337-340.
  • 8CAYOT P, TAINTURIER G. The quantification of protein amino groups by the trinitrobenzenesulfonic acid method: a re-examination [J]. Anal Bioehem, 1997,249(2) : 184-200.
  • 9ZALIPSKY S, SELTZER R, MENON-RUDOLPH S. Evaluation of a new reagent for covalent attachment of polyethylene glycol to proteins[J]. Biotechnol Appl Biochem, 1992,15 (1) : 100-104.

二级参考文献35

  • 1[1]Naito R,Yokoyama K.An improved perflourodecalin emulsion.In: GA Jamieson,TJ Greenwalt,eds.Blood Substitutes and Plasma Expanders,New York: Alan R Liss Inc,1978;81:87
  • 2[2]Wahr JA,Trouwborst RK,Spence RK,et al.A pilot study of the efficiency of an oxygen earrying emulsion Oxygen(TM.In:patients undergoing surgical blood loss Anesthesiology,1995;80:A397
  • 3[3]Goodin T.Results of a phase I clinical trial of a 40 v/v%emulsion of HM351 (OxygflourTM) in healthy volunteers.2nd Annual IBC Conference on Blood Substitute and Related Products,Washington DC,1994
  • 4[4]Riess,Jean G.Blood substitutes and other potential biomedical applications of fluorinated colloids.Journal of Fluorine Chemistry,2002;114(2):119
  • 5[5]Perutz MF.Myoglobin and hemoglobin: role of distal residues in reactions with haem ligands.Trends Biochem Science,1989;14:42
  • 6[6]Benesch R,Benesch RE,Yung S,et al.Hemoglobin covalently bridged across the polyphosphate binding site.Biochem Phys Res Commun,1975;63:1123
  • 7[7]Gould SA,Moss GS.Clinecal development of human polymerized hemoglobin as a blood substitute.World J Surg,1996;20:1200
  • 8[8]Hughes GS,Yancey EP,Albrecht R,et al.Hemoglobin-based oxygen carrier preserves submaximal exercise capacity in humans.Clin Pharmacol Ther,1995;58:434
  • 9[9]ALi AC.Interference of o-raffinose cross-linked hemoglobin in three methods for serum creatini creatinine.Clin Chem,1997;43:1738
  • 10[10]Jia L,Bonaventura C,libavebtrya J,et al.S-nitrosohaemoglobin: a dynamic activity of blood involved in vascular control.Nature,1996;380:221

共引文献12

同被引文献14

引证文献1

二级引证文献2

生物医学工程学杂志
2009年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部