摘要
采用ｐＳＶＰ０Ｍｃａｔ微基因修饰雪旺细胞（ＳＣ）脊髓内移植，观察其对损伤脊髓再生的影响。将ＳＤ大鼠脊髓半横断损伤模型随机分为ｐＳＶＰ０Ｍｃａｔ微基因修饰ＳＣ移植组（Ａ组）、ＳＣ移植组（Ｂ组）及损伤对照组（Ｃ组），每组１０只大鼠。术后３个月，用辣根过氧化物酶（ＨＲＰ）逆行示踪技术及体视学计量法，检查移植术后脊髓的再生能力。结果：①Ａ、Ｂ两组的ＨＲＰ标记细胞在近损伤区上段、红核、动眼神经核等中脑水平均有发现，Ａ组多于Ｂ组，Ｃ组损伤近端未发现ＨＲＰ标记细胞。②损伤区脊髓前角灰质神经元密度及１００μｍ白质范围内有髓神经纤维计数：Ａ组＞Ｂ组＞Ｃ组。提示。
In order to observe the role of genetically modified Schwann cell (SC) with pSVP_0Mcat in the regeneration of injured spinal cord, the cells were implanted into the spinal cord. Ninety SD rats were used to establish a model of hemitransection of spinal cord at the level of T8, and were divided into three groups, randomly, that is, pSVP0Mcat modified SC implantation (Gorup A), SC implantation (Group B) and without cell implantation as control (Goup C). After three months the presence of axonal regeneration of the injured spinal cord was examined by means of horseradish peroxidase (HRP) retrograde labelling technique and stereography. The results indicated that HRP labelled cells in Group A and B could be found in the superior region of injured spinal cord and the brain stem such as the red nuclei and ocluomotor nuclei. The density of ventral horn neurons of the spinal cord and the number of myelinated axons in 100 μm of the white matter was A>B>C group. In brief, the pSVP0Mcat modified SC intraspinal implantation could promote regeneration of the injured spinal cord.
出处
《中国修复重建外科杂志》
CAS
CSCD
1998年第5期257-260,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
神经再生
脊髓损伤
基因修饰
脊髓再生
雪旺细胞
Spinal cord injury
Genetically modified
Implantation
Schwann cell
Regeneration of spinal cord
