摘要
通过ＰＥＧ法及转化脂介导法将不同调控序列控制下的ｇｕｓ基因导入柑桔原生质体中，研究了ｇｕｓ基因在柑桔原生质体中的瞬间表达情况，结果表明，在柑桔原生质体中，控制ｇｕｓ基因表达水平的能力由大至小依次为ＣａＭＶ３５Ｓ启动子、ＡｄｈＩ启动子及ｒｂｃｓ启动子，内含子的存在可以提高ｇｕｓ基因在柑桔原生质体中的表达水平。
Gus genes controlled by several kinds of regulator sequences were introduced into citrus protoplasts respectively by PEG and lipofectinmediated transformation.The transient expression of gus gene in citrus protoplasts had been investigated.The expression level of the citrus protoplasts transformated with plasimid pBI121 and p35SGSINT(both gus genes were controlled by CaMV35S promoter) was higher than that transformated with plasmid pAT13(gus gene controlled by Adhl promoter) and pRGN73(gus gene under control of rice rbcs promoter).It was found that intron increase the expression level in citrus protoplasts.
出处
《福建农业学报》
CAS
1998年第2期1-5,共5页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金
关键词
瞬间表达
GUS基因
调控序列
柑桔
原生质体
Transient expression
Gus gene
Regulator sequenece
Citrus
Protoplasts
