摘要
【目的】对采集自上海、江西、山东、江苏、河南和湖南等省的257份进行了PCV2的检测,确定中国PCV2感染情况及分子特征。【目的】设计一对针对PCV2ORF2的引物,利用PCR对其进行PCV2基因组的检测,对PCV2阳性的样品,进行了ORF2的克隆、序列测定和分析。【结果】结果显示PCV2阳性率为24.51%(63/257),并能从健康猪群中检测的阳性;各PCV2分离株间ORF2核苷酸序列同源性为84.4%～100%,氨基酸同源性为82.5%～100%,与所属群的不同而不同。【结论】中国有2个群的PCV2毒株在流行,以PCV2b群为主(70.97%,22/31),但也有PCV2a群毒株(29.03%,9/31)的流行,同时PCV2流行的基因型没有明显的地域间差异。部分毒株符合高致病性PCV2的特征,暗示中国已存在高致病性PCV2的流行。
[ Objective] A pair of primer was designed for detection of porcine circovirus type 2 from Shanghai, Jiangxi, Shandong, Jiangsu, Henan and Hunan in 2007. [Result] Results indicated that the positive percentage of PCV2 was 24.51% （63/257） and positive samples were detected from healthy pigs. Gene amplificated from PCR product was cloned and sequenced. Sequence analysis illustrated that nucleotide and amino acid homology of ORF2 of PCV2 isolates were 84.4%-100% and 82.5%-100%, respectively, and defferent belong to defferent groups . [Conclusion] There were two groups of PCV2 strains prevailing in China, the PCV2b group strains were predominant prevailing strain （70.97%, 22/31）, but the PCV2a group strains were prevailing also. There was no difference in genotype of PCV2 isolated from defferent geographical regions. Some PCV2 strains owned character of high pathogenic PCV2 implied that there was a high pathogenic PCV2 prevailing in China.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第8期2949-2957,共9页
Scientia Agricultura Sinica
基金
国家“十一五”科技支撑项目(2006BAD06A01)
