摘要
建立了可同时测定VB1、VB2、VB6、VB12和烟酰胺等常见添加B族维生素含量的离子对反相液相色谱法。流动相为甲醇-醋酸（0．5％）（体积比30：70其中每升流动相含5mmol的庚烷磺酸钠和0．05％三乙胺）；流速为1．2mL／min；采用紫外检测器，波长为270nm；色谱柱为ZORBAX Eclipse XDB—C18，柱温为30℃。各种维生素含量测定方法的线性关系良好，R=0．9993～0．9998；回收率96．8％～100．2％，RSD=0．65％～1．43％。本方法应用于市售功能性饮料中B族维生素含量的测定，结果准确。
An accurate and reliable ion - pair reversed - phase method was established for the determination of VB1 , VB2, VB6, VB12 and nicotinamide in functional drinks. These vitamins can be separated on ZORBAX Eclipse XDB - C18 column by using methanol and 0.5% acetic acid （30：70） as mobile phase at the rate of 1.2 ml/min with VWD detector at 270 nm. The calibration curves showed good linearity （96.8% -100. 2% ）. The recovery rate and RSD were 96.8% - 100.2% and 0.65% - 1.43% , respectively. The simple and rapid method was successfully applied to the determination of functional drinks in the market.
出处
《中国酿造》
CAS
北大核心
2009年第8期140-142,共3页
China Brewing
