摘要
从印度洋深海热液区沉积物中筛选到一株耐盐菌，菌株形态呈杆状，长1.5-2.0μ，宽0.5-0.7μm，属革兰氏阴性菌，生长适温为4-55℃，最适为35℃；pH测试范围为4.0-10.5，最适为7.0；测试NaCl浓度为0-5.13mol／L，最适为0.86mol／L，该菌属于兼性厌氧菌。经16S rDNA鉴定该菌属于γ-变形菌纲（γ-Proteobac-teria）盐单胞菌属（Halomonas），命名为Halomonas sp.YD-7。该菌与菌株Halomonas sp.ANT9086在进化位置上最为接近，同源性达98.1％，有可能是新种。对该菌株的生理生化特性进行了研究，发现该菌具有很强的适应能力，对温度、盐度和氧气的适应范围广。该菌株还具有很强的耐受和去除Mn^2＋的能力及一定的耐受和去除Cr^6＋的能力。表明该菌在污水处理与生物修复方面可能具有重要的应用价值。
A strain of Halomonas sp. YD-7 is isolated from the sediments at a deep-sea hydrothermal vent in the Indiam Ocean. It appears about 1.5-2.0μm long, 0.5-0.7μm wide, bacillus-like, and Gram-negative. It grows in a temperature range between 4℃ and 55℃ （optically at 35℃）, in a pH range between 4.0 and 10.0（optically at 7.0）, and in a NaCl concentration range between 0 mol/L and 5.13 mol/L （optically at 0.86 mol/L）. It is a {acuhative anaerobe. By means of the 16s rDNA gene sequences identification, it belongs to Genus Halomonas, in Phylum 7-Proteobacteria, and it is named Halomonas sp. YD-7. It also has about 98.1% of the homogeous similarity to Halomonas sp. ANT9086, indicating that they are close to each other in the evolution tree. Therefore it might be a novel species. In addition, its physiological and biochemical characteristics are studied. It is found that the bacteria have an extensive acclimatization ability in a large range of temperature, salinity, or oxygen concentration. It can survive in the high Mn^2＋ and Cr^6＋ environments, and it is able to remove them from the surroundings. Therefore it might be potentially used for the polluted-water treatment and bioremediation.
出处
《海洋科学进展》
CAS
CSCD
北大核心
2009年第3期367-375,共9页
Advances in Marine Science
基金
中国大洋协会--深海热液区微生物的营养和生存策略研究(DYXM-115-02)
关键词
盐单胞菌
鉴定
生理生化
Halomonas
identification
physiology and biochemistry
