摘要
目的探讨哮喘患者呼出气冷凝液(EBC)中8-异前列腺素(8-iso-PG)水平与病情严重程度的关系,阐明EBC中8-iso-PG监测对评估哮喘气道氧化应激的价值。方法纳入59例哮喘患者和13名健康对照为研究对象。哮喘患者按照病情分为急性发作组15例,非急性发作组44例(轻度间歇者13例,轻度持续者15例,中重度持续者16例)。采用EcoScreen收集EBC,酶联免疫分析法(EIA)检测EBC中的8-iso-PG水平。结果哮喘急性发作组EBC中8-iso-PG水平较非急性发作组高(P<0.01)。非急性发作组中轻度持续[(17.9±1.2)pg/mL]和中重度持续[(39.7±4.0)pg/mL]患者EBC中8-iso-PG水平较健康对照组显著升高(P<0.01),轻度间歇[(13.5±1.1)pg/mL]与健康对照无显著差异(P>0.05)。中重度持续较轻度持续高,轻度持续较轻度间歇高(P<0.01)。轻度持续哮喘患者吸入激素治疗1个月后EBC中8-iso-PG水平[(17.7±1.1)pg/mL]较治疗前无显著变化(P>0.05)。结论EBC中8-iso-PG的增高与哮喘病情严重程度有关,可作为检测哮喘气道氧化应激的客观指标。吸入糖皮质激素后8-iso-PG无显著性降低,提示吸入糖皮质激素不能有效地控制哮喘气道氧化应激损伤。
Objective To invesitgate the relationship between 8-isoprostane （8-iso-PG） level in exhaled breath condensates （EBCs） and severity of asthma and explore the role of 8-iso-PG in asthma evaluation and monitoring. Methods Fifty-nine patients with asthma were enrolled. In which 15 cases were acute exacerbation, 13 cases were mild intermittent, 15 cases were mild persistent, and 16 cases were moderate-to-severe persistent. Thirteen healthy volunteers were recruited as control. EBCs were collected using EcoScreen system. The 8-iso-PG levels in EBCs were measured by a specific enzyme immunoassay. The patients with mild intermittent asthma were treated with inhaled corticosteroid （ICS） for one month and their EBCs were recollected for 8-iso-PG measurement. Results Exhaled 8-iso-PG levels were obviously increased in the patients with acute asthma compared with those chronic asthmatics [ （47.2 ± 6. 8 ） pg/mL vs （24. 5± 12.0） pg/mL,P 〈0. 01 ]. In the chronic persistent asthma,the levels were significantly higher in patients with mild persistent and moderate-to-severe asthma [ （ 17.9 ± 1.2） pg/mL and （39. 7 ±4. 0） pg/mL, P 〈0. 01 ]. While 8-iso-PG level did not differ significantly in intermittent asthma [ （13.5 ±1.1 ） pg/mL] compared with the control subjects （ P 〉 0. 05 ）. After one-month ICS treatment the 8-iso-PG level in the patients with mild intermittent asthma did not change significantly although the ACT score improved. Conclusions 8-iso-PG levels in EBC are associated with the severity of asthma,implicating 8-iso-PG may be useful in monitoring airway oxidative stress in asthma. ICS treatment is incapable of decreasing the 8-iso-PG, suggesting the ICS has minor impact on oxidative stress.
出处
《中国呼吸与危重监护杂志》
CAS
2009年第4期337-340,共4页
Chinese Journal of Respiratory and Critical Care Medicine
基金
云南省应用基础研究面上项目(编号:2008ZC076M)
