摘要
目的观察鞣花酸对前列腺癌PC-3细胞株的影响。方法:体外培养人前列腺癌PC-3细胞,加入0μg/ml、2.5μg/ml、5μg/ml、10μg/ml、20μg/ml的鞣花酸作用于PC-3细胞,分别作用12小时、24小时和48小时后,应用MTT法测定各浓度组鞣花酸对PC-3细胞的生长抑制作用。应用流式细胞仪检测鞣花酸作用48小时后,PC-3细胞的周期时相变化及凋亡情况。应用免疫细胞化学检测10ug/ml鞣花酸作用24小时后,实验组与对照组细胞Caspase-3蛋白表达情况。结果:鞣花酸明显抑制PC-3细胞的生长,抑制效应呈时间依赖型和浓度依赖型,与对照组比较均有统计学意义(P<0.05)。应用流式细胞仪检测结果显示,鞣花酸可将PC-3细胞阻滞于G1/S期,并诱导PC-3细胞凋亡。免疫细胞化学结果显示实验组Caspase-3蛋白表达明显升高。结论:鞣花酸对前列腺癌PC-3细胞株有明显的生长抑制和诱导凋亡作用。
Objective： To observe the effect of Ellagic Acid on prostate cancer cell llne PC - 3. Methods： The human prostate cancer PC -3 cells were treated in vitro with EA in 0μg/ml,2.5μg/ml,5μg/ml, 10μg/ml, 20μg/ml for 12,24 ,48 hours. The cell viability was detected by MTr in all groups. The cell cycle arrest and apoptosis was detected by flow cytometry when PC - 3 cells were treated with EA of diferent conccrtration in the 48 hours. The immunocytochemistry method was used to assess the expression of Caspase - 3 protein when PC - 3 cells were treated with EA 10μg/ml for 24 hours. Results：EA significantly inhibited the pmroliferation and viability of the PC -3 cells in a time and dose dependent manner compared with the untreated control groups （all P 〈 0.05）. Flow cytometric analysis revealed that treatment with EA resulted in cell - cycle arrest in the G1 phase and increasing the percentages of apoptetic ceils in a dose - dependent manner. The immtmocytochemistry showed that Caspase -3 expression was higher compared with control groups, when PC -3 cells were treated with EA. Conclusions：E1- lagic Acid could enhance the growth suppression and induce apoptosis of PC -3 ceils.
出处
《湖北职业技术学院学报》
2009年第2期101-105,共5页
Journal of Hubei Polytechnic Institute
