摘要
目的探讨α-促黑素（α-MSH）对银屑病患者外周血单一核细胞（PBMC）产生TNF-α、IL-10的影响。方法标本取自20例寻常性银屑病患者和10例健康献血者，用不同浓度α-MSH处理经过植物血凝素（PHA）刺激的PBMC，用ELISA法检测PBMC培养上清中TNF—α、IL-10水平，应用荧光实时定量PCR分析PBMC中TNF—α mRNA、IL-10 mRNA的表达。结果银屑病患者PBMC培养上清中TNF—α水平高于正常对照（P〈0．01），IL-10水平低于正常对照（P〈0．01）；各浓度α—MSH均可抑制TNF—α的分泌（P〈0．01），浓度为10^-13mol／L时作用最强（P〈0．01）；各浓度α—MSH均可诱导IL—10的分泌（P〈0．01，P〈0．05），浓度为10^-13mol／L时作用最强（P〈0．01）。浓度为10^-13mol／L的α—MSH下调TNF-α mRNA的表达（P〈0．01）、上调IL-10 mRNA的表达（P〈0．01）。结论α—MSH可调节银屑病患者TNF—α和IL-10产生。
Objective To investigate the effects of alpha-melanocyte-stimulating hormone （alpha-MSH） on the production of tumor necrosis factor-alpha （TNF-alpha） and interleukin-10 （IL-10） by peri-heral blood monohuclear cells （PBMCs） from patients with psoriasis vulgaris. Methods Heparinized peripheral blood was obtained from 20 patients with psoriasis vulgaris and 10 healthy human controls. PBMCs were isolated, cultured in complete medium, and stimulated with phytohemagglutinin （PHA） alone, the combination of PHA and various concentrations of alpha-MSH, or nothing. After another 48-hour culture, ELISA and real-time PCR were performed to measure the secretion levels of TNF-alpha and IL-10 in the supernatants of cultured PBMCs as well as the mRNA expression levels of TNF-alpha and IL-10 in PBMCs. Results The secretion level of TNF-alpha in the supernatants of patient-derived PBMCs stimulated by nothing or PHA alone was significantly higher than that from normal control-derived PBMCs （329.87 ± 99.33 ng/L vs 116.95 ± 37.15 ng/L, 1756.01 ± 183.60 ng/L vs 1287.30 ± 152.36 ng/L, both P〈0.01）. alpha-MSH of all tested concentrations （ 10^-13, 10^-11, 10^-9, 10^-7 mol/L） could inhibit the secretion of TNF-alpha by PBMCs compared with PHA alone （all P 〈 0.01 ）, and the maximum effective concentration was 10^-13 mol/L. On the contrary, a significant decrease was observed in the secretion level of IL-10 in the supematants of patientderived PBMCs stimulated by nothing or PHA alone compared with normal control-derived PBMCs （P 〈 0.05 or 0.01 ）. Moreover, the secretion of IL-10 by PBMCs was promoted by alpha-MSH of all tested concentrations （P 〈 0.01 or 0.05）, with the maximum effective concentration being 10^-13 mol/L （P 〈 0.01 ）. The alpha-MSH of 10^-13 mol/L down-regulated the mRNA expression of TNF-alpha （P 〈 0.001 ）, but up-regulated that of IL-10 （P 〈 0.001 ） in PHA-stimulated PBMCs from patients. Conclusion alpha-MSH can regulate the production of TNF-alpha and IL-10 by PHA-stimulated PBMCs from patients with psoriasis vulgaris.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2009年第7期477-480,共4页
Chinese Journal of Dermatology
