摘要
目的观察存活素、caspase-3在不对称BPH中的表达情况，探讨凋亡在BPH发病机制中的作用。方法采用自身对照设计，应用PV二步法免疫组织化学方法检测25例不对称BPH增生明显叶与不明显叶中存活素、caspase-3的表达情况。结果增生明显叶组存活素蛋白阳性率为（22．08±16．33）％，不明显叶组为（7．64±5．45）％，2组间差异有统计学意义（t=4．817，P〈0．01）。增生明显叶组caspase-3蛋白阳性率为（33．12±21．01）％，不明显叶组为（51．52±27．27）％，2组间差异有统计学意义（t=-5．770，P〈0．01）。两者在增生明显叶组表达呈负相关（r=-0．474，P〈0．05）；而在增生不明显叶组表达无相关性（r=-0．313，P〉0．05）。结论存活素、caspase-3引起腺体的不同凋亡状态与前列腺增生程度密切相关，提示凋亡机制在BPH的发生与发展中发挥重要作用。
Objective To compare the different expression patterns of survivin and caspase-3 between the larger part and the smaller part of the dissymmetric enlarged prostates in BPH patients, and to clarify the role of apoptosis in BPH etiology. Methods BPH tissues from 25 patients were embedded in paraffin. The specimens constituted of two prostate parts from each patient. One part was from the larger enlarged gland and the other was from the smaller enlarged counterpart. 50 paraffin blocks were cut into sections and stained with survivin and caspase-3 polyclonal antibodies against human. All the data was analyzed by SPSS13.0 software. Results Survivin immunoreactivity was localized in prostatic cell nuclei. Survivin expression in larger enlarged parts （22.08± 16.33）% was significantly higher than the smaller parts （7.64±5.45）% （P〈0.01）. Positive nuclear or cytoplasm staining was also observed in the Caspase-3 immunoreactivity. Caspase-3 expression was higher in smaller enlarged parts （51. 52 ± 27. 27%） than in larger enlarged parts （33. 12 ± 21. 01%） （P〈 0.01）. Survivin expression in larger part of the enlarged prostates was inversely correlated with caspase-3 expression （r=- 0. 474, P〈0.05）. However, in smaller part of the enlarged prostates, the expression of the two factors had no relation （r=-0. 313,P〉0.05）. Conclusions Survivin and caspase-3 expression patterns are different in the two parts of the dissymmetric enlarged prostates. These different patterns indicate apoptosis playing an important role in BPH development.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2009年第7期484-486,共3页
Chinese Journal of Urology
