摘要
目的探讨胆固醇酯转运蛋白基因(CETP)TaqIB位点多态性对健康青年血脂比值的影响及在高糖低脂(HC/LF)膳食诱导的血脂比值变化中的作用。方法56名健康青年志愿者,给予7d的平衡膳食和6d的HC/LF膳食,平衡膳食蛋白、脂肪、碳水化合物含量分别为15%、31%、54%,HC/LF膳食分别为15%、15%、70%。于第1d、第8d及第14d取空腹血,测定血脂水平,计算甘油三酯/高密度脂蛋白胆固醇(TG/HDL-C)、log(TG/HDL-C)、总胆固醇(TC)/HDL-C、低密度脂蛋白胆固醇(LDL-C)/HDL-C值。分离基因组DNA,以聚合酶链反应-限制性酶切法分析CETPTaqIB位点多态性。分析不同基因型间血脂比值的差异。结果不同基因型受试者血脂比值的基础值无明显差异。平衡膳食后,男性组B2等位基因携带者TC/HDL-C值比B1B1纯合子明显降低(P<0.05),而在女性组没发现差异。HC/LF膳食后,无论是在受试人群整体还是将男女分组分析,不同基因型之间血脂比值均无明显差异。与HC/LF膳食前相比,在受试人群整体,B1B1基因型受试者4个血脂比值均发生明显改变(P<0.05),而B2等位基因携带者仅LDL-C/HDL-C和TC/HDL-C明显降低(P<0.05)。HC/LF膳食前后4个血脂比值的差值在各基因型之间没有明显差异。男女分组分析发现,HC/LF膳食后TG/HDL-C和log(TG/HDL-C)较膳食前升高(P<0.05)仅在女性B1B1基因型受试者中出现;而LDL-C/HDL-C和TC/HDL-C均明显下降(P<0.05),且不受性别和CETPTaqIB位点多态性的影响。4个血脂比值在HC/LF膳食前后的差值,仅TC/HDL-C在男性B1B1受试者与B2携带者之间存在明显差异(P<0.05)。结论CETPTaqIB位点多态性在健康青年男性影响HC/LF膳食诱导的TC/HDL-C改变,在女性TG/HDL-C和log(TG/HDL-C)的升高中也有一定作用。
Objective To investigate the effects of cholesterol ester transfer protein gene （CETP） TaqlB polymorphism on serum lipid ratios and their responses to high-carbohydrate/low-fat （HC/LF） diet in healthy youth. Methods After on regular diet for 7 days, 56 health youth C（22.89± 1.80） years] were given HC/LF diet for 6 days. The regular diet contained 54% carbohydrate, 15% protein, and 31% fat of the total energy. The LF/ HC diet contained 70% carbohydrate, 15% protein, and 15% fat of the total energy. The serum lipids were measured on the 1^st, the 8^th and the 14^th day. The ratios of TG/HDL-C, log （TG/HDL-C）, TC/HDL-C, and LDL- C/HDL-C were calculated. The polymorphism of CETP was analyzed by PCR-RFLP method. Results No significant difference was found of the lipid ratios at baseline in subjects with different genotypes in both genders. After the regular diet, TC/HDL-C was significantly lower in males carrying the B2 allele than BIB1 homozygotes （P〈0.05）. After HC/LF diet, no significant difference was found of the lipid ratios between the subjects with different genotypes in the whole study population or in males and females separately. Compared with those before HC/LF diet, subjects with B1B1 genotype experienced significant changes of the four lipid ratios after HC/LF diet （P〈0.05）, while the B2 carriers had only significant decrease of TC/HDL-C and LDL-C/HDL-C （P〈0.05）. No significant difference of the changes of the four lipid ratios before and after HC/LF diet was found between the subjects with different genotypes. When gender was taken into account, significant increases of TG/HDL-C and log （TG/HDL-C） were found in females with BIB1 genotype （P〈0.05）. All the subjects experienced significant decrease of TC/HDL-C and LDL-C/HDL-C regardless of gender and genotype （P〈0.05）. As for the changes of lipid ratios before and after HC/LF diet, difference of only TC/HDL-C was found between males with B1B1 and male carriers of B2 （P〈0. 05）. Conclusion Polymorphism of CETP TaqIB may influence the response of TC/ HDL-C to HC/LF diet in males and the increases of TG/HDL-C and log （TG/HDL-C） induced by HC/LF diet in females.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2009年第4期684-688,共5页
Journal of Sichuan University(Medical Sciences)
基金
教育部新世纪优秀人才支持计划(项目编号:NCEF04-0863)
四川省科技计划(项目编号:2008HH0008)资助
关键词
胆固醇酯转运蛋白
基因多态性
高糖低脂膳食
血脂比值
Cholesterol ester transfer protein Gene polymorphism High-carbohydrate/low-fat diet Lipid ratios
