摘要
在ABTS法检测漆酶酶活性的基础上对黑孢漆斑菌的生长条件进行摸索.采用生长曲线分析,正交多因素分析及单因素分析对初始酸碱度,培养时间,接种量,菌龄,摇床转速及光照条件进行研究.结果表明:最佳培养条件为初始pH5.5,接种量7.5%,菌龄9天,摇床转速160 r/min,自然光照,培养9天.
Based on ABTS assay of laccase activity, the growth conditions of Myrothecium melanosporum is explored. The result is discussed based on growth curve analysis, orthogonal multi-factor analysis and single factor analysis of the initial pH, culture time, inoculu, concentration fungus age ,velocity of shaker and light conditions. The results show that： the best culture conditions is initial pH 5.5, culture time 9 days, velocity of shaker 160 r/min, natural light.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2009年第3期111-113,共3页
Journal of Central South University of Forestry & Technology
基金
林业公益性行业科研项目(200804001)
云南省发改高科项目(2007-1718)
关键词
生物化学
漆斑菌
黑孢漆斑菌
漆酶
生长条件
Biochemistry
Myrothecium
Myrothecium melanosporum
laccase
growth condition
