摘要
介绍了固定金属离子亲和色谱(IMAC)吸附蛋白质的基本原理、蛋白质吸附与解吸的影响因素以及洗脱过程中需要注意的问题,并简述了IMAC今后研究的方向。
The basic principle of immobilized metal ion affinity chromatography (IMAC), the factors affected adsorption and desorption of protein in IMAC, and the problems need attention during desorption were introduced, as well as the future research direction of IMAC.
出处
《中国酿造》
CAS
北大核心
2009年第7期4-7,共4页
China Brewing
基金
河南省基础与前沿研究计划(072300440060)
关键词
固定金属离子亲和色谱
蛋白质
吸附
洗脱
immobilized metal ion affinity chromatography
protein
adsorption
desorption