摘要
对苦参进行驯化栽培是解决其供需矛盾的主要途径,也是保护生态环境最有效的措施。本试验研究氮肥对苦参生长、生物总碱含量和生物总碱产量的效应。采用盆栽试验,施纯氮量设0,50,100,150和200 mg/kg五个水平,完全随机设计。结果表明,施用氮肥对苦参生长以及苦参总碱含量均有显著促进效应。各施氮处理根干重均与对照差异显著,当施纯氮150 mg/kg时根干重最高,比对照增加42%;根直径随施氮量提高呈增加趋势,当施纯氮150 mg/kg时根直径最大,比对照增加21.1%;叶绿素总含量在施纯氮100 mg/kg时最高,比对照增加33.8%;苦参总碱含量在施纯氮100 mg/kg时最高,比对照增加35.7%;单株苦参总碱产量在施纯氮150 mg/kg时最高,较对照增加90.5%。氮对根干重增加效应主要是由于根直径增加引起根体积增加所致,苦参叶片中叶绿素含量随施氮量增加有利于苦参总碱含量提高。
The effect of nitrogen on growth, total alkaloids concentration and total alkaloids yield of Sophora flavescens was studied. Four levels of N （50, 100, 150, 200 mg/kg） were applied using urea （N 46%） in a pot experiment. The control was no N. Plant height, root diameter, root dry weight, aerial dry weight, Chl concentration and total alkaloids concentration increased relative to the amount of N applied. The root dry weight was 26. 7 g/plant at 150 mg/kg N applied, an increase of 42% compared with the control. Root dry weight/ root fresh weight and root dry weight/total plant dry weight decreased with amount of N applied. Nitrogen application had significant positive effect on root diameter. The root diameter was highest at 150 mg/kg N applied, an increase of 21.1% compared with the control. The percentage of xylem diameter increased with N applied while the percentage of root bark depth decreased. Chl concentration and total alkaloids concentration increased with N applied. Chl concentration was highest at 100 mg/kg N applied, an increase of 33.8% compared with the control. Total alkaloids concentration （4.4%） was also highest at 100 mg/kg N applied, an increase of 35.7 % compared with the control. Total alkaloids concentration increased by improving Chl concentration. The total alkaloids yield per plant （1.18 g/plant） was highest at 150 mg/kg N applied, an increase of 90.5 % compared with the control.
出处
《草业学报》
CSCD
北大核心
2009年第3期159-164,共6页
Acta Prataculturae Sinica
基金
甘肃省科技攻关项目(2GS064-A43-019-08)资助
关键词
氮肥
苦参
苦参总碱
叶绿素
生长
nitrogen
Sophora flavescens
total alkaloids
Chl
growth
