摘要
为了解ＥＢ病毒在中线Ｔ细胞淋巴瘤中的感染情况，作者对合肥地区４６例中线Ｔ细胞淋巴瘤进行ＥＢＶ检测，用聚合酶链反应检测ＥＢＶ特征性的ＤＮＡ序列（ＥＢＶＤＮＡ），用ＲＮＡ原位杂交法检测ＥＢＶ编码的ＲＮＡ（ＥＢＥＲ１／２）。结果：ＥＢＶＤＮＡ阳性率为７６．１％（３５／４６）；ＥＢＥＲ１／２阳性率为６７．４％（３１／４６）。鼻腔、鼻咽、口咽Ｔ淋巴瘤ＥＢＥＲ１／２阳性率分别为８８．９％（１６／１８）、７１．４％（５／７）、４７．６％（１０／２１）。在多形细胞性淋巴瘤中，中多形和大多形细胞性ＥＢＥＲ１／２阳性率为８０．０％（１６／２０），小多形细胞性为７３．３％（１１／１５）。结果表明ＥＢＶ感染与中线Ｔ淋巴瘤关系密切，它在该肿瘤的发病中可能起重要作用，中线Ｔ淋巴瘤的ＥＢＶ感染率与解剖学部位有关，多形细胞性淋巴瘤ＥＢＶ感染率较高。
To observe the infection of Epstein Barr virus (EBV) in midline T cell lymphoma(MTL),46 cases of MTL were studied.Expression of EBV specific DNA(EBVDNA) and EBV encoded RNA(EBER1/2) were detected with polymerase chain reaction (PCR) and RNA in situ hybridization (ISH RNA).The positive rate of EBVDNA was 76.1%(35/46).Positive rate of EBER1/2 was 67.4%(31/46).The positive rates of EBER1/2 in the nasal,nasopharygeal and pars pharyngoorale were 88.9%(16/18).71.4%(5/7) and 47.6%(10/21) respectively.In pleomorphic lymphoma the EBV positive rate of middle and large cell type was 80.0%(16/20),in small cell type it was 73.3%(11/15).The results suggest that MTL is strong associated with EBV,it might play an important role in the pathogenesis of MTL.The infectious frequency of EBV in MTL is related to anatomical sites.Positive rate of EBV in pleomorphic lymphoma were higher especially in middle and large cell type.
出处
《实用癌症杂志》
1998年第2期121-123,共3页
The Practical Journal of Cancer
关键词
淋巴瘤
中线T细胞
MTL
E-B病毒
RNA
原位杂交
Midline T cell lymphoma Epstein Barr virus Polymerase chain reaction RNA in situ hybridization
