摘要
从福建泉州华侨大学林园采集的土样中分离出一株高产胞外黑色素的放线菌(Actinomycetes)G-HD-4。将黑色素从该菌株发酵液中提取纯化后经红外光谱、紫外光谱以及扫描电镜的分析,与标准黑色素基本一致。通过形态特征、培养特征以及理化性质比较发现该放线菌属于链霉菌属,根据多项分类原则和系统进化树的构建分析,分离的菌G-HD-4与卡胞链霉菌(Streptomyces capoamus)处于同一分支上,序列相似性达到99%以上,因此将该菌株暂归入卡胞链霉菌。
Actinomyceter strain G-HD-4 was isolated from soft sample collected from Huaqiao flower garden in Quanzhou of Fujian Province, which produced the most melanin compared to other isolated 18 strains. The melanin separated from the fermentation broth of strain G-HD-4 was characterized by ultraviolet-visible （UV-Vis） spectroscopy, infrared （IR） spectroscopy, and scanning electron microscope （SEM）. The results revealed that the melanin derived from strain G-HD-4 was similar to standard melanin on the structural basis. Based on the studies of physiological and biochemical characteristics and 16S ribosomal RNA sequence homology, the G-HD-4 strain was confirmed to belong to Streptomyces genus and be similar to Streptomyces capoamus （gene sequence similarity 99%）. Finally, it was identified as a species of Streptomyces capoamus by taxonomic and phylogentic analysis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第11期180-184,共5页
Food Science
