摘要
用ＰＣＲ方法扩增人表皮生长因子（ＥＧＦ）片段，并将其与绿脓杆菌外毒素（ＰＥ４０）片段分别插入质粒ｐＥＴ－１７ｂ中，经酶切分析和ＰＣＲ检测证明成功地构建了表达质粒ｐＥＧＦ４０，旨在表达一种对癌细胞有特异杀伤作用的融合蛋白。
oth fragments of amplified epidermal growth factor (EGF) BY PCR and pseudomonas aeruginosa exotoxin (PE40) wee inserted into expression plasmid pET17b.Plasmid pEGF40 was constructed successfully and identified by enzyme digestion analysis and PCR technique.The purpose is expressing fusion protein which can kill cancer cells specially and trying to get a new kind of targetting drug.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第3期182-183,共2页
Chinese Journal of Immunology
