摘要
目的利用MRI观察超顺磁性纳米颗粒(SPIO)标记的膜联蛋白V(Annexin V)体外检测凋亡细胞的能力。方法对5管不同的细胞进行MRI和普鲁士蓝染色,分别为:A管:未经H2O2诱导凋亡处理的细胞与SPIO孵育;B管:经H2O2诱导凋亡处理的细胞与SPIO孵育;C管:经H2O2诱导凋亡处理的细胞的空白管;D管:经H2O2诱导凋亡处理的细胞与Annexin V-SPIO孵育;E管:未经H2O2诱导凋亡处理的细胞与Annexin V-SPIO孵育。结果5管间总体比较MR信号值差异有统计学意义(F=569.38,P<0.01),其中A、B、C三管的MR信号值差异无统计学意义,D和B管、E和A管、D和E管的MR信号值比较差异均有统计学意义。普鲁士蓝染色显示Annexin V-SPIO可与凋亡细胞膜特异性结合。结论Annexin V-SPIO可与体外凋亡细胞特异性结合,并引起磁共振信号改变。
Objective To evaluate the feasibility of imaging apoptotic cells in vitro by MRI with SPIO labeled Annexin V.Methods Five tubes(A-E)containing different cells were scanned with MR and then stained with Prussian blue,including tube A(cells not treated with H2O2 before incubated with SPIO),tube B(cells treated with H2O2 before incubated with SPIO),tube C(cells not treated with H2O2 and as control tube),tube D(cells treated with H2O2 before incubated with Annexin V-SPIO)and tube E(cells not treated with H2O2 before incubated with Annexin V-SPIO).Results There were statistically significant differences among five tubes on the signal intensity of MR(F=569.38,P<0.01),especially between tube D and tube B,tube E and tube A,tube D and tube E.Prussian blue stain revealed SPIO labeled Annexin V could bind specifically to apoptotic cells.Conclusion SPIO labeled Annexin V can bind specifically to apoptotic cells,inducing the signal intensity changes of MRI.
出处
《中国医学影像技术》
CSCD
北大核心
2009年第5期734-737,共4页
Chinese Journal of Medical Imaging Technology
关键词
磁共振成像
凋亡
膜联蛋白V
Magnetic resonance imaging
Apoptosis
Annexin V
