摘要
目的:研究苇茎Phragmites communis Trin.的化学成分。方法:采用有机溶剂提取, 反复硅胶柱色谱和重结晶法进行分离纯化, 根据化合物的理化性质和波谱数据鉴定其结构; 以A549肿瘤细胞株为研究对象, 采用 MTT 法对所分得的化合物进行体外抗肿瘤活性评价。结果:从苇茎乙醇提取物中分离得到 14 个化合物, 分别鉴定为:金色酰胺醇酯(aurantiamide acetate, 1)、2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone (2)、阿魏酸(ferulic acid, 3)、对香豆酸(p-coumaric acid, 4)、丁香酸(syringic acid, 5)、香草酸(vanillic acid, 6)、对羟基苯甲酸(p-hydroxybenzoic acid, 7)、对羟基苯甲醛(p-hydroxybenzaldenhyde, 8)、棕榈酸(palmitic acid, 9)、十七烷酸(heptadecanoic acid, 10)、β-谷甾醇(β-sitosterol, 11)、豆甾醇(stigmasterol, 12)、α-D-葡萄糖(α-D-glucose, 13)和β-D-葡萄糖(β-D-glucose, 14), 它们对A549肿瘤细胞生长无明显抑制活性。结论:化合物1和2为首次从芦苇属植物中分得, 化合物4、5、7、8 和10为首次在该种植物中得到。
AIM： To study the chemical constituents from the rhizomas of Phragmites communis Trin.. METHODS： The constituents were isolated by column chromatography, and their structures were elucidated by phys- ico-chemical properties and spectroscopic analysis. The inhibitory effects of these compounds on A549 cell lines were tested in vitro by MTT methods. RESULTS： Fourteen compounds were isolated and identified as aurantiamide acetate （1）, 2,3-dihydroxy-l-（4-hydroxy-3,5-dimethoxyphenyl）-l-propanone （2）, ferulic acid （3）, p-coumaric acid （4）, syfingic acid （5）, vanillic acid （6）, p-hydroxy benzoic acid （7）, p-hydroxybenzaldenhyde （8）, palmitic acid （9）, heptadecanoic acid （10）, β-sitosterol （11）, stigmasterol （12）, α-D-glucose （13） and t-D-glucose （14）. CONCLUSION： Compounds 1 and 2 were obtained from Phragmites genus for the first time; compounds 4, 5, 7, 8 and 10 were isolated from P. com- munis for the first time. These compounds did not show obvious cancer cell growth inhibition against the A549 cell lines.
出处
《中国天然药物》
SCIE
CAS
CSCD
北大核心
2009年第3期196-198,共3页
基金
supported by National 11th Five-Year scientific and Technological Support Projects (No. 2006BAI11B08-01)
2006"Qinglan Project"for Training Sci-entific and Technological Innovation Team for Jiangsu’s College~~
关键词
苇茎
禾本科
化学成分
Phragmites communis
Gramineae
Constituents
