摘要
目的:研究静脉给予大鼠人参皂苷Rg2后,其在胆汁、粪便和尿液中的排泄。方法:采用反相高效液相色谱(HPLC)-紫外检测器(UVD)法测定大鼠胆汁、粪便和尿液中的人参皂苷Rg2;Dikma Diamonsil TMC18色谱柱(4.6 mm×250 mm,5μm),以甲醇-4%磷酸水溶液(65∶35)为流动相,检测波长为203 nm。结果:HPLC-UVD测定方法的标准曲线线性关系、样品回收率和日内、日间精密度均符合生物样品分析要求。给大鼠静脉注射人参皂苷Rg2后,5.5 h内胆汁中原形人参皂苷Rg2累积排泄量为给予剂量的27.2%,24 h内粪便中原形人参皂苷Rg2累积排泄量为给予剂量的22.6%;尿液中未检出人参皂苷Rg2。结论:静脉给予大鼠人参皂苷Rg2,原形药物主要通过胆汁和粪便途径排出体外。
Objective： To determine the ginsenoside Rg2 and study its excretion in bile, feces and urine of rat. Method： Reversed phase high-performance liquid chromatographic （RP-HPLC） method with an ultra-violet detector （UVD） was performed at a detection wavelength of 203 nm and with a Dikma Diamonsil^TM C18 column （4. 6 mm ×250mm,5μm）, which the mobile phase was consisted of methanol-aq. 4% H3PO4 （65：35 ）, for determination of the ginsenoside Rg2 in bile, feces and urine after administration of the ginsenoside Rg2 to rat at a tail vein single dose of 20 mg · kg^-1. Result： The HPLC-UVD method fulfilled all the standard requirements of linearity, recovery, precision, and accuracy. After tail vein administration of the ginsenoside Rg2 to rat, the 5.5 hour cumulative biliary excretion rate and the 2d-hour cumulative feces excretion rate of intact ginsenoside Rg2 were 27.2% and 22. 6% of the administered dose, respectively. But intact ginsenoside Rg2 could not be detected in urine during this experimental period. Conclusion： The bile and feces were the main excretion routs of the unchanged form after tail vein administration of the ginsenoside Rg2 to rat.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第10期1281-1284,共4页
China Journal of Chinese Materia Medica
基金
国家科技部“1035”工程项目(96-901-01-12)
关键词
人参皂苷RG2
药物排泄
高效液相色谱
ginsenoside Rg2
drug excretion
high-performance liquid chromatography
