摘要
目的:观察大鼠心肌缺血/再灌注损伤对血清和心肌组织瘦素(Leptin)表达的影响,探讨Leptin在心肌缺血/再灌注损伤中的作用。方法:建立大鼠心肌缺血/再灌注模型,检测血清乳酸脱氢酶(LDH)和Leptin浓度,并用HE染色和免疫组织化学观察心肌组织病理学及Leptin表达水平。结果:缺血组、再灌注组血清LDH水平显著升高(P<0.05),表明该模型制作成功,造成心肌局部一定程度的损伤。缺血组血清Leptin含量(6.34±2.49)ng/ml显著低于对照组(7.50±2.93 ng/ml,P<0.05);再灌注后Leptin水平缓慢恢复,于再灌注2 h时Leptin达到(8.32±1.74)ng/ml,恢复到损伤前水平(8.38±2.56)ng/ml,且随再灌注时间延长有升高趋势。免疫组化显示与假手术组心肌Leptin蛋白表达水平相比,其他四组均有显著降低(P<0.01),按缺血45 min后再灌注1 h组、缺血45min后再灌注3 h组、单纯缺血45 min组、缺血45 min后再灌注2 h组依次递减。结论:Leptin在心肌缺血/再灌注损伤后早期45 min血中有明显减少,心肌组织中也明显表达下降。心肌组织病理损伤与Leptin的改变可能有一定的关系。
Aim： To explore the effect of rat myocardial ischemia/reperfusion injury on leptin levels in serum and myocardium, and discuss the role of leptin in myocardial ischemia/reperfusion injury. Methods： A myocardial ischemia/reperfusion injury model of rats was estabilished, senan lactate dehydrogenase（LDH） and leptin levels were detected, and histopathological changes and leptin expressions in myocardium were investigated by hematoxylin-eosin staining and immunohistochemistry, respectively. Results： Serum LDH of ischemia and reperfusion groups increased significantly（P 〈 0.05）, suggesting the model was successfully established and a certain degree of local myocardial injury was induced. Serum leptin of ischemia group（6.34 ± 2.49） ng/ml was significantly lower than control group（7.50 ± 2.93 ng/ml, P 〈 0.05）. Leptin levels recovered gradually after reperfusion, reached（8.32 ± 1.74）ng/ml at 2 h after repeffusion, which recovered to the level before injury （8.38 ± 2.56）ng/nd, and showed a trend to increase as reperfusion time was elongated. Immunohistochemistry results showed that as compared with sham-operation group, myocardial leptin protein expressions of the other four groups were all significantly lower（ P 〈 0.01 ）, and decreased in order by 45 min ischemia/1 h reperfusion, 45 min ischemia/3 h reperfusion, 45 min ischemia and 45 min ischemia/2 h reperfu±sion. Conclusion： Leptin level in the blood decreases significantly at the early 45 min after myocardial ischemia/reperfusion injury, and its expression in myocardium also decreases significantly. There may be a certain relationship between the pathological injury of myocardium and the changes of leptin.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2009年第2期221-223,I0003,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金(30670821)
关键词
瘦素
缺血/再灌注损伤
心肌
乳酸脱氢酶
leptin
ischemia/repfersion injury
myocardium
lactate dehydrogenase
