摘要
以玉米幼叶生长部位为材料，通过水溶性多聚物ＰＥＧ４０００／ＤｅｘｔｒａｎＴ５００构成的两相系统，对玉米幼叶生长部位质膜进行了分离。结果表明，由ＰＥＧ４０００６．２％、ＤｅｘｔｒａｎＴ５００６．２％、ＫＣｌ５ｍｍｏｌ／Ｌ、磷酸缓冲液ｐＨ７．８４ｍｍｏｌ／Ｌ、蔗糖８．５％构成的两相系统最适宜分离玉米幼叶生长部位质膜。经过三次相分配后，质膜在上相中的分配率达８５％以上。经－０．４ＭＰａ胁迫处理２４ｈ后的材料在上述两相系统中分配时，其质膜、叶绿体膜、线粒体膜在上相中的分配率均稍有下降，内质网膜、液泡膜则稍有增加，但这些变化都不太显著，并且质膜在上相中的分布占绝对优势。
The Plasma Membranes (PM) of maize young leaves growth zone were isolated by the method of two phase partition.The results showed that the best composition of two phase system which isolated PM was:Dextran T500 6.2%,PEG 4000 6.2%,K phosphate buffer pH 7.8 4 mmol/L,KCl 5 mmol/L and sucrose 8.5%.After three times phase partition,the partition of PM in the upper phase was beyond 85%.When maize seedlings were stressed by -0.4 MPa for 24 hour,the partition of PM and other internal membranes in the upper phase took some changes,but the changes were not obvious,and PM was in advantage of the upper phase absolutely.
出处
《西北植物学报》
CAS
CSCD
1998年第1期83-89,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金
关键词
两相法
质膜
玉米
纪叶
生长部位
two phase partition,plasma membrane,maize young leaves growth zone,water stress
