摘要
提出了用间接原子吸收法测定黄连素制剂中盐酸小檗碱含量的新方法。基于在酸性介质中和室温时，盐酸小檗碱与〔（Ｃｏ（ＳＣＮ）〕２－形成稳定的离子对缔合物，在０．５１μｇ／ｍＬ～５．８０μｇ／ｍＬ范围内，吸光度与浓度呈线性关系，相关系数为０．９９９８，该方法的检测限为０．０２μｇ／ｍＬ，变异系数为１．５％（ｎ＝１１）。用于黄连素制剂的测定，平均回收率为（９９．２±１．６）（ＳＤ）％（ｎ＝１２）。
A new atomic absorption spectrometric method for the indirect determination of berberini hydrochloridum in berberine perparation was proposed. The method is based on which berberini hydrochloridum and Co(SCN) 4 2- form a stable ion pair associate complex in citric acid medium (pH=2.0) at normal temperature. The calibration curve is linear over the range of 0.51 mg/mL～5.80 mg/mL ( r =0.999 8). the detection limit is 0.02 μg/mL, and the coefficient of variation is 1.5% ( n =11), the method has been used to determine berberini hydrochloridum in the pharmaceutical preparations, and the average recovery is (99.2±1.6)( SD )%, In the new method, the result of determination is the same as description in Chinese Medicine Dictionary.
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第2期123-125,130,共4页
Journal of Northwest University（Natural Science Edition）
