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多重PCR检测社区获得性肺炎患者中的病原体 被引量：2

Detection of Pathogens in Patients with Community-acquired Pneumonia by Multiplex PCR
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摘要 目的评价多重PCR在快速诊断社区获得性肺炎病原体中的作用。方法提取痰液标本中的DNA,扩增病原体DNA后电泳并观察其结果,同时给予普通PCR或分离培养作为对照。结果成功检测出32例感染者,与普通PCR符合率达100%,敏感性高于分离培养。结论多重PCR能高通量、快速诊断病原体,值得临床进一步推广。 Objective To observe the rapid diagnosis value of multiplex PCR in patients with community-acquired pneumonia. Methods DNA was extracted and amplified from sputum. Common PCR or separation culhure was used as control. Results Pathogens were amplified from 32 patients,compared with common PCR,the coincidence was 100%,but the sensitivity was higher than separation culhure. Conclusion Multiplex PCR is a high-flux, repid method for clinical diagnosis of pathogens.
作者 何新平 刘竞芳 谭慧敏
机构地区 长沙市中心医院老干科
出处 《中国现代医生》 2009年第12期57-58,共2页 China Modern Doctor
关键词 多重PCR 社区获得性肺炎 病原体 Multiplex PCR Community-acquired pneumonia Pathogen
分类号 R563.1 [医药卫生—呼吸系统]
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参考文献8

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共引文献3119

同被引文献22

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中国现代医生
2009年 第12期

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