摘要
目的探讨抗核周因子(antiperinuclear factor,APF)、抗角蛋白(anti-RA keratin antibody,AKA)、抗环瓜氨酸肽(anticyclic citrullinated peptide antibodies,CCP)联合检测在类风湿关节炎(rheumatoid arthritis,RA)诊断和鉴别诊断中的临床应用价值。方法RA患者119例,非RA患者101例及健康对照组39例。酶联免疫吸附法定量检测抗CCP;免疫荧光法检测抗-AKA、抗APF,并采用四格表法计算敏感度及特异性。结果RA组抗-APF、抗AKA、抗-CCP的敏感度分别为48.8%、32.3%、83.5%;特异性分别为92.2%、93.1%、94.1%。结论抗APF、抗-AKA、抗-CCP 3种自身抗体对RA具有高度特异性,且在RA早期即可出现。3种抗体联合检测有助于类风湿关节炎诊断和鉴别诊断。
Objective To explore the clinical value of combined detection of antiperinuelear factor （APF）,anti-RA keratin antibody （AKA） and anticyclic citrullinated peptide antibody （anti-CCP） in diagnosis and differential diagnosis of rheumatoid arthritis （RA）. Methods Totally 119 RA patients,101 non-RA rheumatisants and 39 healthy controls were enrolled in the study. Enzyme linked immunosorbent assay （ELISA） was applied to quantitately detecting anti-CCP antibody; irnmunofluorescence method was applied to quantitately determining AKA and APF. Then the quadruple tabular form was used to calculate the sensitivity and specificity. Results In RA group,the sensitivity of APF, AKA and anti-CCP antibody was 48.8% ,32.3% and 83.5% respectively; the specificity was 92.2%, 93.1% and 94.1 % respectively. Conclusion Three autoantibodies,including APF,AKA and anti-COP antibody have high specificity in early diagnosis of RA. Combined detection of the three antibodies contributes to diagnosis and differential diagnosis of rheumatoid arthritis （RA）.
出处
《国际检验医学杂志》
CAS
2009年第4期339-341,共3页
International Journal of Laboratory Medicine
关键词
关节炎
类风湿
自身抗体
抗体
抗核
临床实验室技术
Arthritis, rheumatoid
Autoantibodies
Antibodies, antinuclear
Clinical Laboratory Techniques
