摘要
[目的]探讨细胞因子信号转导抑制分子-(1SOCS-1)基因启动子区甲基化在胃癌发生和发展过程中的意义。[方法]收集南京市原发性胃癌患者102例为胃癌组;采用1∶1病例-对照研究方法,随机选取非消化系统疾病、非肿瘤患者102例为对照组。取外周血提取DNA,采用甲基化特异性PCR检测胃癌组及对照组外周血DNASOCS-1基因启动子区甲基化状态。[结果]胃癌组SOCS-1基因启动子区甲基化率为38.24%(39/102),对照组为12.75%(13/102)。两组OR=4.2381(95%CI:2.0924￣8.5840)。两组中年龄及性别亚组间的甲基化率差异均无统计学意义(P>0.05)。[结论]SOCS-1基因启动子区异常甲基化可能是肿瘤特异性的,在胃癌的发生、发展中可能具有一定的作用。
[Objective] To explore relationship between aberrant methylation status of promoter of suppressor of cytokine signaling-1 （ SOCS-1 ） gene and gastric cancer. [ Methods ] Methylation-specific PCR （ MSP ） technique were used to detect the methylation status of promoter of SOCS-1 among 102 cases of gastric cancer and an 1 ： 1 control group of cancer-free and matched by age（±5 years） and sex. Each subject was blood sampled for analyzing.[Results ] The rate of SOCS-1 gene promoter aberrant methylation in gastric cancer was 38.24%（ 39/102 ）, while in corresponding controls was 12.75%（ 13/102 ）, and the OR between case group and control group is 4.2381（95%CI： 2.0924-8.5840 ）. There was no significant difference between the two groups in age and sex sub-group（P〉0.05）. [Conclusion] High frequent hypermethylation of SOCS-1 is mostly cancer specific, and it may play a role in the development of gastric cancer.
出处
《环境与职业医学》
CAS
北大核心
2009年第2期121-124,共4页
Journal of Environmental and Occupational Medicine
基金
东南大学科研项目基金(编号:XJ019004)
