摘要
以笃斯越桔新生嫩芽为外植体，应用均匀设计法筛选其最适合的基部直接再生芽苗和生根的培养基，结果表明，最适合的基部直接再生芽苗诱导培养基为：DR＋2-ip 2．75mg·L^-1＋IAA0．10mg·L^-1，诱导率为99％；生根培养基：MS（改良）＋IAA 1．00mg·L^-1＋Kt0．30mg·L^-1，生根率达98％；以再生植株的茎节为材料进行快繁的结果表明，在25d的一个培养周期内增殖倍数平均达40以上，快繁培养基：MS（改良）＋IAA0．50～1．00mg·L^-1＋Kt0．30mg·L^-1＋GA3 0．20mg·L^-1。建立了笃斯越桔的植株再生和快繁体系。
The tender buds of Vaccinium uliginosum were used as explants for the experiment. Uniform Design was applied to select the most suitable media for shoots regeneration at base of tender buds and rooting. The results showed that DR ＋2-ip 2.75mg·L^-1 ＋ IAA 0.10 mg·L^-1 fitted for shoots regeneration at base of tender buds, the rate of regeneration was 99% ; MS（modified） ＋ IAA 1.00mg·L^-1＋ Kt 0.30 mg·L^-1 for rooting, the rate of rooting was 98% ; Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 40-fold proliferation rate was achieved within 25 days, MS（modified） ＋ IAA 0.50 - 1.00 mg·L^-1 ＋ Kt 0.30 mg·L^-1 ＋ GA3 0. 20mg·L^-1 for rapid propagation of plant regeneration. Plant Regeneration and Rapid Propagation system of Vaccinium uliginosum has been successfully established.
出处
《林业科学研究》
CSCD
北大核心
2009年第2期226-229,共4页
Forest Research
基金
通化师范学院自然科学基金资助项目(XS060074)
吉林省科技厅资助项目(200705C05)
关键词
笃斯越桔
离体培养
植株再生
均匀设计
Vaccinium uliginosum
in vitro culture
plant regeneration
Uniform Design
