摘要
目的建立金黄色葡萄球菌随机扩增ＤＮＡ多态性（ＲＡＰＤ）分型技术，以应用于临床菌种鉴定及流行病学调查。方法对临床分离的１５株金黄色葡萄球菌，酚氯仿法提取其ＤＮＡ后行ＲＡＰＤ分型，同时进行噬菌体分型。结果１５株临床分离株大多数产生独特而稳定的ＲＡＰＤ带型，可分成５个噬菌体型和１４个ＲＡＰＤ谱型。采用同一反应体系，产肠毒素金黄色葡萄球未出现ＲＡＰＤ谱型。结论ＲＡＰＤ基因分型技术不需已知核酸序列，分型率高，分辨力强，简便快捷，可为金黄色葡萄球菌临床分离株提供分型标志。
おo establish the random amplification of polymorphic DNA(RAPD)typing method of S.aureus and to apply this in the clinical strain identification and epidemiology investigation.Methods\ DNA from 15 clinical strains of S.aureus were extracted by the phenol chloroform method and used in the RAPD assay,phage typing was also performed.Resutls\ Almost all the clinical strains showed stable and distinct RAPD pattern and were divided into 5 phagetypes and 14 RAPD profiles,while enterotoxin producint S.aureus failed to show RAPD pattern using this assay.Conclusions \ RAPD provides markers for the typing of clinical strains and is suitable for molecular epidemiologic studies with high typeability,powerful discrimination,simplicity and rapidness.
出处
《卫生研究》
CAS
CSCD
北大核心
1998年第S1期22-24,共3页
Journal of Hygiene Research
关键词
金黄色葡萄球菌
DNA多态性
噬菌体
\ Staphylococcus aureus, random amplified polymorphic DNA,phage
